概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to MEKK1
  • 经测试应用
    适用于: ICC/IF, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment: SKNSMTLDLN SSSKCDDSFG CSSNSSNAVI PSDETVFTPV EEKCRLDVNT ELNSSIEDLL EASMPSSDTT VTFKSEVAVL SPEKAENDDT YKDDVNHNQK , corresponding to amino acids 1211-1310 of Human MEKK1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab212601 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab55653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
  • 序列相似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIM-type zinc finger.
  • 翻译后修饰
    Autophosphorylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • M3K1_HUMAN antibody
    • MAP3K1 antibody
    • MAPK/ERK kinase kinase 1 antibody
    • MAPKKK1 antibody
    • MEK kinase 1 antibody
    • MEKK 1 antibody
    • Mekk antibody
    • MEKK1 antibody
    • Mitogen activated protein kinase kinase kinase 1 antibody
    • Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody
    • Mitogen-activated protein kinase kinase kinase 1 antibody
    • SRXY6 antibody
    see all

图片

  • Western blot against tagged recombinant protein immunogen using ab55653 MEKK1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • ab55653 staining MEKK1 in murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 4°C and then incubated with ab55653 at a 1/200 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/1000 dilution.

    See Abreview

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (Hela)
Specification
Hela
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cultured Cells (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Jun 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Loading amount
20 µg
Specification
C2C12
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 02 2011

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepa1-6 (murine hepatoma))
Loading amount
40 µg
Specification
Hepa1-6 (murine hepatoma)
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Platelets)
Specification
Platelets
Preparation
Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy
Platelets
Username

Abcam user community

Verified customer

提交于 Dec 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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