Anti-MEK7 (phospho S271 + T275)抗体(ab4762)

概述

  • 产品名称
    Anti-MEK7 (phospho S271 + T275)抗体
    参阅全部 MEK7 一抗
  • 描述
    兔多克隆抗体to MEK7 (phospho S271 + T275)
  • 特异性
    This antibody does not react with other MEK isoforms.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide (Human) derived from a region of human MEK 7 that contains serine 271 and threonine 275. The sequence is conserved in mouse.

  • 阳性对照
    • Fusion protein expressing MEK 7.

性能

应用

Our Abpromise guarantee covers the use of ab4762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 38 kDa.

靶标

  • 功能
    Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.
  • 组织特异性
    Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
  • 序列相似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Activated by phosphorylation on Ser/Thr.
  • 细胞定位
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c-Jun N-terminal kinase kinase 2 antibody
    • Dual specificity mitogen activated protein kinase kinase 7 antibody
    • Dual specificity mitogen-activated protein kinase kinase 7 antibody
    • JNK activating kinase 2 antibody
    • JNK kinase 2 antibody
    • JNK-activating kinase 2 antibody
    • JNKK 2 antibody
    • Jnkk-2 antibody
    • Jnkk2 antibody
    • MAP kinase kinase 7 antibody
    • MAP2K7 antibody
    • MAPK/ERK kinase 7 antibody
    • MAPKK 7 antibody
    • MAPKK-7 antibody
    • MAPKK7 antibody
    • MEK 7 antibody
    • Mitogen Activated Protein Kinase kinase 7 antibody
    • MKK 7 antibody
    • MKK-7 antibody
    • MKK7 antibody
    • MP2K7_HUMAN antibody
    • PRKMK 7 antibody
    • PRKMK-7 antibody
    • PRKMK7 antibody
    • SAPK kinase 4 antibody
    • SAPKK-4 antibody
    • SAPKK4 antibody
    • Sek 2 antibody
    • Sek-2 antibody
    • Sek2 antibody
    • SKK4 antibody
    • stress-activated protein kinase kinase 4 antibody
    see all

Anti-MEK7 (phospho S271 + T275) antibody 图像



  • Predicted band size : 38 kDa


    Peptide Competition: Extracts prepared from background extracts with tagged fusion protein expressing MEK 7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4762 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine containing peptide (3), a generic phosphothreonine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method.

    Phosphatase Stripping: Extracts were prepared as above. Membranes were left untreated (6, 8) or treated (7, 9) with lambda phosphatase, then incubated with 0.50 µg/mL ab4762 antibody (6, 7), or MEK 7 pan antibody (8, 9) for two hours at room temperature, and developed using the same procedure as above. The data show that only the peptide corresponding to ab4762 blocks the antibody signal, and that phosphatase stripping eliminates only the phospho- signal, thereby demonstrating the specificity of the antibody. Note that the tagged fusion protein expressing MEK 7 runs at  65 kDa.

实验方案

Anti-MEK7 (phospho S271 + T275) antibody (ab4762)参考文献

ab4762 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Fibroblast)
Loading amount
30 µg
Specification
Fibroblast
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Feb 05 2009

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