Anti-MEK6 (phospho S207)抗体(ab36722)

概述

  • 产品名称Anti-MEK6 (phospho S207)抗体
    参阅全部 MEK6 一抗
  • 描述
    兔多克隆抗体to MEK6 (phospho S207)
  • 特异性This antibody detects endogenous levels of MKK6 only when phosphorylated at Serine207.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Synthetic phosphopeptide derived from human MKK6 around the phosphorylation site of Serine 207 (V-D-SP-V-A).

  • 阳性对照
    • Breast carcinoma tissue and MDA-MB-435 cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab36722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
  • 组织特异性Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Weakly autophosphorylated.
    Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • 数据库链接
  • 形式It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
  • 别名
    • Dual specificity mitogen activated protein kinase kinase 6 antibody
    • Dual specificity mitogen-activated protein kinase kinase 6 antibody
    • MAP kinase kinase 6 antibody
    • MAP2K6 antibody
    • MAPK/ERK kinase 6 antibody
    • MAPKK 6 antibody
    • MAPKK6 antibody
    • MEK 6 antibody
    • MEK6 antibody
    • Mitogen Activated Protein Kinase Kinase 6 antibody
    • MKK 6 antibody
    • MKK6 antibody
    • MP2K6_HUMAN antibody
    • PRKMK6 antibody
    • Protein kinase mitogen activated kinase 6 antibody
    • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody
    • SAPK kinase 3 antibody
    • SAPKK-3 antibody
    • SAPKK3 antibody
    • SKK3 antibody
    • Stress-activated protein kinase kinase 3 antibody
    see all

Anti-MEK6 (phospho S207) antibody 图像

  • Immunohistochemical analysis of paraffin embedded breast carcinoma, using ab36772 diluted 1:50. Left: Untreated Ab; Right: Ab pre-treated with synthesized phosphopeptide.
  • All lanes : Anti-MEK6 (phospho S207) antibody (ab36722) at 1/500 dilution

    Lane 1 : Extracts from untreated MDA-MB-435 cells
    Lane 2 : Extracts from UV treated MDA-MB-435 cells

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab36722 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36722, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MEK6 (phospho S207) antibody (ab36722)参考文献

ab36722 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 45 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hela)
Specification Hela
Treatment rhIL-6 (100 ng/ml) for 30 min
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 06 2014

My apologies for the delay, I asked the laboratory if they had further suggestions, and they were unable to help more, however they stated that they have not tried Hela cells, only MDA-MB- 435 cells treated with UV. They have however noticed that the t...

Read More

Thank you for providing your protocol details, I believe that it is very good and have have only a few suggestions to make: - you do not detail how long you incubated the primary antibodies for: I would recommend doing an overnight incubation, if you...

Read More

Thank you for your enquiry. I am sorry you are experiencing problems with two of our antibodies; as you can detect total levels of the proteins this suggests that your extraction protocol is adequate; it could be that the problem lies with the anti...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"