使用敲除细胞株进行验证RabMAb

Anti-MEK6抗体[EP557Y] (ab33866)

概述

  • 产品名称Anti-MEK6抗体[EP557Y]
    参阅全部 MEK6 一抗
  • 描述
    兔单克隆抗体[EP557Y] to MEK6
  • 特异性ab33866 recognises MKK6.
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in the N terminal of human MKK6.

  • 阳性对照
    • Hela cell lysate
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab33866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC.
  • 靶标

    • 功能Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
    • 组织特异性Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Weakly autophosphorylated.
      Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • 数据库链接
    • 形式It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
    • 别名
      • Dual specificity mitogen activated protein kinase kinase 6 antibody
      • Dual specificity mitogen-activated protein kinase kinase 6 antibody
      • MAP kinase kinase 6 antibody
      • MAP2K6 antibody
      • MAPK/ERK kinase 6 antibody
      • MAPKK 6 antibody
      • MAPKK6 antibody
      • MEK 6 antibody
      • MEK6 antibody
      • Mitogen Activated Protein Kinase Kinase 6 antibody
      • MKK 6 antibody
      • MKK6 antibody
      • MP2K6_HUMAN antibody
      • PRKMK6 antibody
      • Protein kinase mitogen activated kinase 6 antibody
      • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody
      • SAPK kinase 3 antibody
      • SAPKK-3 antibody
      • SAPKK3 antibody
      • SKK3 antibody
      • Stress-activated protein kinase kinase 3 antibody
      see all

    Anti-MEK6 antibody [EP557Y] 图像



    • Predicted band size : 37 kDa

      Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)

      Lanes 2, 6 and 10: MEK6 knockout HAP1 cell lysate (20 µg)

      Lanes 3, 7 and 11: HeLa cell lysate (20 µg)

      Lanes 4, 8 and 12: A431 cell lysate (20 µg)

      Lanes 1, 2, 3 and 4: Green signal from target – ab33866 observed at 37 kDa

      Lanes 5, 6, 7 and 8: Red signal from loading control – ab18058 observed at 130 kDa

      Lanes 9, 10, 11 and 12: Merged (red and green) signal

      ab33866 was shown to specifically react with MEK6 when MEK6 knockout samples were used. Wild-type and MEK6 knockout samples were subjected to SDS-PAGE. ab33866 and ab18058 (loading control to Vinculin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-MEK6 antibody [EP557Y] (ab33866) at 1/10000 dilution + Hela cell lysate

      Predicted band size : 37 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    Anti-MEK6 antibody [EP557Y] (ab33866)参考文献

    ab33866 has not yet been referenced specifically in any publications.

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