产品名称 Anti-MEK6抗体 参阅全部 MEK6 一抗
特异性 This antibody is specific for MKK6.
WB, IHC-P, ELISA more details
A synthesized non phosphopeptide derived from human MKK6 around the phosphorylation site of Serine 207.
WB: MDA-MB-435 cells
IHC-P: Breast carcinoma tissue
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Corresponding phospho antibody
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Use at an assay dependent concentration.
功能 Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
组织特异性 Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
序列相似性 Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.
翻译后修饰 Weakly autophosphorylated. Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Information by UniProt
形式 It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
Dual specificity mitogen activated protein kinase kinase 6 antibody
Dual specificity mitogen-activated protein kinase kinase 6 antibody
MAP kinase kinase 6 antibody
Anti-MEK6 antibody 图像
Western blot - MKK6 antibody (ab47593)
All lanes : Anti-MEK6 antibody (ab47593) at 1/500 dilution Lane 1 : Extracts from MDA-MB-435 cells Lane 2 : Extracts from MDA-MB-435 cells, preincubated with synthesized
peptide Predicted band size : 37 kDa Observed band size : 37 kDa
Immunohistochemistry (Paraffin-embedded sections) - MKK6 antibody (ab47593)
Immunohistochemical analysis of paraffin embedded breast carcinoma tissue, using ab47593 at a 1/50 dilution and Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)secondary antibody.
Left image: Untreated.
Right image: Treated with synthesized peptide.
Anti-MEK6 antibody (ab47593)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"