Anti-MEK5抗体[EP648Y] (ab45146)


  • 产品名称Anti-MEK5抗体[EP648Y]
    参阅全部 MEK5 一抗
  • 描述
    兔单克隆抗体[EP648Y] to MEK5
  • 经测试应用适用于: WB, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues within human MEK5.

  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab45146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ICC/IF: 1/100 - 1/250.

    WB: 1/10000. Predicted molecular weight: 49 kDa.

    Is unsuitable for IHC, FACS or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
    • 组织特异性Expressed in many adult tissues. Abundant in heart and skeletal muscle.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 OPR domain.
      Contains 1 protein kinase domain.
    • 结构域Binds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A.
    • 翻译后修饰Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
      Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Dual specificity mitogen activated protein kinase kinase 5 antibody
      • Dual specificity mitogen-activated protein kinase kinase 5 antibody
      • EC antibody
      • HsT17454 antibody
      • MAP kinase kinase 5 antibody
      • MAP kinase kinase MEK5b antibody
      • MAP2K5 antibody
      • MAPK/ERK kinase 5 antibody
      • MAPKK 5 antibody
      • MAPKK5 antibody
      • MEK 5 antibody
      • mitogen-activated protein kinase kinase 5 antibody
      • MKK5 antibody
      • MP2K5_HUMAN antibody
      • PRKMK5 antibody
      • Protein kinase, mitogen-activated, kinase 5 antibody
      • SAPKK5 antibody
      • SKK5 antibody
      see all

    Anti-MEK5 antibody [EP648Y] 图像

    • Anti-MEK5 antibody [EP648Y] (ab45146) at 1/10000 dilution + HeLa cell lysate

      Predicted band size : 49 kDa
      Observed band size : 49 kDa
    • Immunofluorescent staining of HeLa using ab45146.
    •  ab45146 at 1/100 staining HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with goat serum prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 488 conjugated goat polyclonal antibody was used as the secondary. This antibody detects MEK5 in HeLa cells as a cytoplasmic protein.

      See Abreview

    Anti-MEK5 antibody [EP648Y] (ab45146)参考文献

    ab45146 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. I am sorry you are experiencing difficulty with this antibody. Typically when there are black dots on the blot, the antibody is binding with the blocking agent and it is necessary to filter the blocking agent. Was the same ...

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    I understand your concerns about the quality of the antibody. I can confirm, that we would expect the antibody to work well. However, if you experience any problems with this product please do not hesitate and come...

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    I can confirm that ab45146 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on ...

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    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa, passage 78)
    Loading amount 20 µg
    Specification HeLa, passage 78
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

    Abcam user community

    Verified customer

    提交于 Apr 13 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa, passage 78)
    Specification HeLa, passage 78
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 3%

    Abcam user community

    Verified customer

    提交于 Apr 05 2007