RabMAb

Anti-MEK4 (phospho S80)抗体[EP1075Y] (ab52958)

概述

  • 产品名称Anti-MEK4 (phospho S80)抗体[EP1075Y]
    参阅全部 MEK4 一抗
  • 描述
    兔单克隆抗体[EP1075Y] to MEK4 (phospho S80)
  • 经测试应用适用于: IHC-P, WB, IPmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminal of human MKK4

  • 阳性对照
    • 3T3 cell lysates (untreated or treated with PDGF) and human muscle tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab52958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
    • 组织特异性Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
    • Information by UniProt
    • 数据库链接
    • 别名
      • c Jun N terminal kinase kinase 1 antibody
      • C-JUN N-terminal kinase kinase 1 antibody
      • Dual specificity mitogen activated protein kinase kinase 4 antibody
      • Dual specificity mitogen-activated protein kinase kinase 4 antibody
      • JNK Activated Kinase 1 antibody
      • JNK activating kinase 1 antibody
      • JNK-activating kinase 1 antibody
      • JNKK antibody
      • JNKK1 antibody
      • MAP kinase kinase 4 antibody
      • Map2k4 antibody
      • MAPK ERK kinase 4 antibody
      • MAPK/ERK kinase 4 antibody
      • MAPKK 4 antibody
      • MAPKK4 antibody
      • MEK 4 antibody
      • MEK4 antibody
      • Mitogen activated protein kinase kinase 4 antibody
      • MKK 4 antibody
      • MKK4 antibody
      • MP2K4_HUMAN antibody
      • PRKMK4 antibody
      • SAPK ERK kinase 1 antibody
      • SAPK/ERK kinase 1 antibody
      • SAPKK 1 antibody
      • SAPKK1 antibody
      • SEK1 antibody
      • SERK1 antibody
      • SKK1 antibody
      • Stress activated protein kinase kinase 1 antibody
      see all

    Anti-MEK4 (phospho S80) antibody [EP1075Y] 图像

    • ab52958 at 1/50 dilution staining MKK4 in human muscles by Immunohistochemistry, Paraffin embedded tissue.
    • All lanes : Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958) at 1/5000 dilution

      Lane 1 : 3T3 cell lysate untreated
      Lane 2 : 3T3 cell lysate treated with PDGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 44 kDa
      Observed band size : 44 kDa

    Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958)参考文献

    ab52958 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 60 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Human Cell lysate - whole cell (HepG2)
    Specification HepG2
    Treatment EGF 50ng/ml for 2hrs
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    提交于 Nov 17 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"