概述

  • 产品名称
    Anti-MEK4 (phospho S80)抗体
    参阅全部 MEK4 一抗
  • 描述
    兔多克隆抗体to MEK4 (phospho S80)
  • 特异性
    ab39403 detects endogenous levels of SEK1 / MKK4 only when phosphorylated at Serine 80.
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide: THSpIE, corresponding to amino acids 78 - 82 of Human SEK1/ MKK4

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab39403 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab39403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
  • 组织特异性
    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • 序列相似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c Jun N terminal kinase kinase 1 antibody
    • C-JUN N-terminal kinase kinase 1 antibody
    • Dual specificity mitogen activated protein kinase kinase 4 antibody
    • Dual specificity mitogen-activated protein kinase kinase 4 antibody
    • JNK Activated Kinase 1 antibody
    • JNK activating kinase 1 antibody
    • JNK-activating kinase 1 antibody
    • JNKK antibody
    • JNKK1 antibody
    • MAP kinase kinase 4 antibody
    • Map2k4 antibody
    • MAPK ERK kinase 4 antibody
    • MAPK/ERK kinase 4 antibody
    • MAPKK 4 antibody
    • MAPKK4 antibody
    • MEK 4 antibody
    • MEK4 antibody
    • Mitogen activated protein kinase kinase 4 antibody
    • MKK 4 antibody
    • MKK4 antibody
    • MP2K4_HUMAN antibody
    • PRKMK4 antibody
    • SAPK ERK kinase 1 antibody
    • SAPK/ERK kinase 1 antibody
    • SAPKK 1 antibody
    • SAPKK1 antibody
    • SEK1 antibody
    • SERK1 antibody
    • SKK1 antibody
    • Stress activated protein kinase kinase 1 antibody
    see all

图片

  • Anti-MEK4 (phospho S80) antibody (ab39403) at 1/1000 dilution + Whole cell lysates prepared from mouse small intestine epithelial cells at 30 µg

    Secondary
    HRP conjugated goat polyclonal to rabbit IgG at 1/2500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa


    Exposure time : 20 seconds

    Small intestinal epithelial cells were isolated from mice and 30ug was run onto a 9% denaturing gel. The membrane was incubated with ab39403 (1/1000) washed and then incubated with a goat anti-rabbit secondary antibody. As predicted a 44 kDa band was observed.

    See Abreview

  • ab39403, at a 1/500 dilution, staining human SEK1 / MKK4 in breast carcinoma tissue by Immunohistochemistry, Paraffin embedded tissue.ab39403, at a 1/500 dilution, staining human SEK1 / MKK4 in breast carcinoma tissue by Immunohistochemistry, Paraffin embedded tissue.
  • ab39403, at a 1/500 dilution, staining human SEK1 / MKK4 in breast carcinoma tissue (treated with synthesized phosphopeptide) by Immunohistochemistry, Paraffin embedded tissue.ab39403, at a 1/500 dilution, staining human SEK1 / MKK4 in breast carcinoma tissue (treated with synthesized phosphopeptide) by Immunohistochemistry, Paraffin embedded tissue.

文献

This product has been referenced in:
  • Mason JA  et al. Oncogenic Ras differentially regulates metabolism and anoikis in extracellular matrix-detached cells. Cell Death Differ 23:1271-82 (2016). Read more (PubMed: 26915296) »
  • Kim MJ  et al. Negative regulation of SEK1 signaling by serum- and glucocorticoid-inducible protein kinase 1. EMBO J 26:3075-85 (2007). Read more (PubMed: 17568772) »

See all 2 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Small Intestinal Epithelial cells)
Loading amount
30 µg
Specification
Small Intestinal Epithelial cells
Gel Running Conditions
Reduced Denaturing (9 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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提交于 Dec 15 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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