Anti-MEK4 (phospho S257)抗体(ab61112)

概述

  • 产品名称
    Anti-MEK4 (phospho S257)抗体
    参阅全部 MEK4 一抗
  • 描述
    兔多克隆抗体to MEK4 (phospho S257)
  • 特异性
    ab61112 detects endogenous levels of MEK4 only when phosphorylated at serine 257.
  • 经测试应用
    适用于: IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) derived from around the phosphorylation site of serine 257 (V-D-SP-I-A).

  • 阳性对照
    • Human brain tissue

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab61112 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab61112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ELISA 1/40000.

靶标

  • 功能
    Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
  • 组织特异性
    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • 序列相似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c Jun N terminal kinase kinase 1 antibody
    • C-JUN N-terminal kinase kinase 1 antibody
    • Dual specificity mitogen activated protein kinase kinase 4 antibody
    • Dual specificity mitogen-activated protein kinase kinase 4 antibody
    • JNK Activated Kinase 1 antibody
    • JNK activating kinase 1 antibody
    • JNK-activating kinase 1 antibody
    • JNKK antibody
    • JNKK1 antibody
    • MAP kinase kinase 4 antibody
    • Map2k4 antibody
    • MAPK ERK kinase 4 antibody
    • MAPK/ERK kinase 4 antibody
    • MAPKK 4 antibody
    • MAPKK4 antibody
    • MEK 4 antibody
    • MEK4 antibody
    • Mitogen activated protein kinase kinase 4 antibody
    • MKK 4 antibody
    • MKK4 antibody
    • MP2K4_HUMAN antibody
    • PRKMK4 antibody
    • SAPK ERK kinase 1 antibody
    • SAPK/ERK kinase 1 antibody
    • SAPKK 1 antibody
    • SAPKK1 antibody
    • SEK1 antibody
    • SERK1 antibody
    • SKK1 antibody
    • Stress activated protein kinase kinase 1 antibody
    see all

Anti-MEK4 (phospho S257) antibody 图像

  • ab61112, at a 1/50 dilution, staining MEK4 in paraffin embedded human brain tissue by Immunohistochemistry with (+) or without (-) the immunising phospopeptide.

Anti-MEK4 (phospho S257) antibody (ab61112)参考文献

ab61112 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Small Intestinal Epithelial)
Loading amount
20 µg
Specification
Small Intestinal Epithelial
Gel Running Conditions
Reduced Denaturing (9% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jan 06 2009

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