概述

  • 产品名称Anti-MEK4抗体[EP615Y]
    参阅全部 MEK4 一抗
  • 描述
    兔单克隆抗体[EP615Y] to MEK4
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in the C term of MEK4 (Human)

  • 阳性对照
    • Hela cell lysate, lung adenocarcinoma
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • 纯化说明ab33912 is a purified IgG.
  • 克隆单克隆
  • 克隆编号EP615Y
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab33912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 44 kDa.
Flow Cyt 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC or IP.
  • 靶标

    • 功能Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
    • 组织特异性Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
    • Information by UniProt
    • 数据库链接
    • 别名
      • c Jun N terminal kinase kinase 1 antibody
      • C-JUN N-terminal kinase kinase 1 antibody
      • Dual specificity mitogen activated protein kinase kinase 4 antibody
      • Dual specificity mitogen-activated protein kinase kinase 4 antibody
      • JNK Activated Kinase 1 antibody
      • JNK activating kinase 1 antibody
      • JNK-activating kinase 1 antibody
      • JNKK antibody
      • JNKK1 antibody
      • MAP kinase kinase 4 antibody
      • Map2k4 antibody
      • MAPK ERK kinase 4 antibody
      • MAPK/ERK kinase 4 antibody
      • MAPKK 4 antibody
      • MAPKK4 antibody
      • MEK 4 antibody
      • MEK4 antibody
      • Mitogen activated protein kinase kinase 4 antibody
      • MKK 4 antibody
      • MKK4 antibody
      • MP2K4_HUMAN antibody
      • PRKMK4 antibody
      • SAPK ERK kinase 1 antibody
      • SAPK/ERK kinase 1 antibody
      • SAPKK 1 antibody
      • SAPKK1 antibody
      • SEK1 antibody
      • SERK1 antibody
      • SKK1 antibody
      • Stress activated protein kinase kinase 1 antibody
      see all

    Anti-MEK4 antibody [EP615Y] 图像



    • Predicted band size : 44 kDa

      Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
      Lanes 2, 6 and 10: MEK4 knockout HAP1 cell lysate (20 µg)
      Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
      Lanes 4, 8 and 12: A431 cell lysate (20 µg)
      Lanes 1, 2, 3 and 4: Green signal from target - ab33912 observed at 44 kDa
      Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
      Lanes 9, 10, 11 and 12: Merged (red and green) signal
      ab33912 was shown to specifically react with MEK4 when MEK4 knockout samples were used. Wild-type and MEK4 knockout samples were subjected to SDS-PAGE. ab33912 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Overlay histogram showing K562 cells stained with ab33912 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33912, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-MEK4 antibody [EP615Y] (ab33912) at 1/1000 dilution + HeLa cell lysate

      Predicted band size : 44 kDa
      Observed band size : 44 kDa
    • IHC-P of lung adenocarcinoma using anti-MKK4 (C term) ab33912 at 1/100.

    Anti-MEK4 antibody [EP615Y] (ab33912)参考文献

    This product has been referenced in:
    • Wuestefeld T  et al. A Direct in vivo RNAi screen identifies MKK4 as a key regulator of liver regeneration. Cell 153:389-401 (2013). IF, IHC ; Mouse . Read more (PubMed: 23582328) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (LnCap, PC3, HeLa cells)
    Loading amount 10 µg
    Specification LnCap, PC3, HeLa cells
    Gel Running Conditions Non-reduced Denaturing
    Blocking step Sigma-AldrichWestern Blocker Solution (W0138) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
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    提交于 Nov 01 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"