概述

  • 产品名称Anti-MEK4抗体
    参阅全部 MEK4 一抗
  • 描述
    兔多克隆抗体to MEK4
  • 特异性ab39648 detects endogenous levels of total SEK1/MKK4 protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (non-phosphorylated) derived from Human SEK1/MKK4 around the phosphorylation site of Serine 80.

  • 阳性对照
    • NIH/3T3 cell extracts.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab39648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA 1/20000.

靶标

  • 功能Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
  • 组织特异性Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c Jun N terminal kinase kinase 1 antibody
    • C-JUN N-terminal kinase kinase 1 antibody
    • Dual specificity mitogen activated protein kinase kinase 4 antibody
    • Dual specificity mitogen-activated protein kinase kinase 4 antibody
    • JNK Activated Kinase 1 antibody
    • JNK activating kinase 1 antibody
    • JNK-activating kinase 1 antibody
    • JNKK antibody
    • JNKK1 antibody
    • MAP kinase kinase 4 antibody
    • Map2k4 antibody
    • MAPK ERK kinase 4 antibody
    • MAPK/ERK kinase 4 antibody
    • MAPKK 4 antibody
    • MAPKK4 antibody
    • MEK 4 antibody
    • MEK4 antibody
    • Mitogen activated protein kinase kinase 4 antibody
    • MKK 4 antibody
    • MKK4 antibody
    • MP2K4_HUMAN antibody
    • PRKMK4 antibody
    • SAPK ERK kinase 1 antibody
    • SAPK/ERK kinase 1 antibody
    • SAPKK 1 antibody
    • SAPKK1 antibody
    • SEK1 antibody
    • SERK1 antibody
    • SKK1 antibody
    • Stress activated protein kinase kinase 1 antibody
    see all

Anti-MEK4 antibody 图像

  • Anti-MEK4 antibody (ab39648) at 1/3000 dilution + Mouse small intestinal epithelial whole cell lysates at 15 µg

    Secondary
    Goat anti-rabbit polyclonal antibody at 1/7000 dilution
    Developed using the ECL technique

    Predicted band size : 44 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa (possible non-specific binding).

    Exposure time : 40 seconds

    This image is courtesy of an anonymous abreview.

    Blocking: 5% milk for 1 hour at 25°C. Dilutent: TBS-TWEEN, incubated for 12 hours at 4°C.

    See Abreview

  • All lanes : Anti-MEK4 antibody (ab39648) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extracts 5-30µg/lane
    Lane 2 : NIH/3T3 cell extracts 5-30µg/lane with blocking peptide at 1 µg/ml


    Predicted band size : 44 kDa
    Observed band size : 44 kDa

Anti-MEK4 antibody (ab39648)参考文献

ab39648 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Small Intestinal Epithelial)
Loading amount 15 µg
Specification Small Intestinal Epithelial
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Mar 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"