使用敲除细胞株进行验证RabMAb

Anti-MEK3抗体[EP555Y] (ab40836)

概述

  • 产品名称Anti-MEK3抗体[EP555Y]
    参阅全部 MEK3 一抗
  • 描述
    兔单克隆抗体[EP555Y] to MEK3
  • 特异性This antibody is specific for MEK3.
  • 经测试应用适用于: IP, WB, Flow Cytmore details
    不适用于: ICC or IHC
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in the N-terminus of Human MEK3.

  • 阳性对照
    • Jurkat, Hela, MCF-7 and K562 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab40836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/50.
WB 1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for ICC or IHC.
  • 靶标

    • 功能Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
    • 组织特异性Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
    • 疾病相关Note=Defects in MAP2K3 may be involved in colon cancer.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated.
      Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
      Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AW212142 antibody
      • dual specificity mitogen activated protein kinase kinase 3 antibody
      • Dual specificity mitogen-activated protein kinase kinase 3 antibody
      • MAP kinase kinase 3 antibody
      • map2k3 antibody
      • MAPK ERK kinase 3 antibody
      • MAPK/ERK kinase 3 antibody
      • MAPKK 3 antibody
      • MAPKK3 antibody
      • MEK 3 antibody
      • MEK3 antibody
      • Mitogen activated protein kinase kinase 3 antibody
      • MKK 3 antibody
      • MKK3 antibody
      • mMKK3b antibody
      • MP2K3_HUMAN antibody
      • PRKMK 3 antibody
      • PRKMK3 antibody
      • protein kinase, mitogen-activated, kinase 3 antibody
      • SAPK kinase 2 antibody
      • SAPKK 2 antibody
      • SAPKK2 antibody
      • Stress activated protein kinase kinase 2 antibody
      see all

    Anti-MEK3 antibody [EP555Y] 图像



    • Predicted band size : 39 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MEK3knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab40836 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab40836 was shown to recognize MEK3 when MEK3 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK3 knockout samples were subjected to SDS-PAGE. ab40836 and ab8245 (loading control to GAPDH) were both diluted to 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-MEK3 antibody [EP555Y] (ab40836) at 1/20000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Hela cell lysate
      Lane 3 : MCF-7 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 39 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    Anti-MEK3 antibody [EP555Y] (ab40836)参考文献

    ab40836 has not yet been referenced specifically in any publications.

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