The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 44 kDa).
功能Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
疾病相关Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.
翻译后修饰MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Dual specificity mitogen activated protein kinase kinase 2 antibody
Dual specificity mitogen-activated protein kinase kinase 2 antibody
ERK activator kinase 2 antibody
MAP kinase kinase 2 antibody
MAPK / ERK kinase 2 antibody
MAPK/ERK kinase 2 antibody
MAPKK 2 antibody
MEK 2 antibody
Microtubule associated protein kinase kinase 2 antibody
Mitogen activated protein kinase kinase 2 antibody
Mitogen activated protein kinase kinase 2 p45 antibody
MKK 2 antibody
PRKMK 2 antibody
Anti-MEK2 antibody [1A1] 图像
Western blot - Anti-MEK2 antibody [1A1] (ab70590)
Predicted band size : 44 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: MEK2 knockout HAP1 cell lysate (20 µg) Lane 3: Jurkat cell lysate (20 µg) Lane 4: K562 cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab70590 observed at 44 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab70590 was shown to recognize MEK2 when MEK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK2 knockout samples were subjected to SDS-PAGE. ab70590 and ab181602 (loading control to GAPDH) were diluted 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - MEK2 antibody [1A1] (ab70590)
All lanes : Anti-MEK2 antibody [1A1] (ab70590) at 1/2000 dilution
Lane 1 : MCF-7 cell lysate Lane 2 : U937 cell lysate Lane 3 : Jurkat T cell lysate Lane 4 : K562 cell lysate