Anti-MEK1 (phospho T286)抗体[EP2364Y] (ab68140)


  • 产品名称Anti-MEK1 (phospho T286)抗体[EP2364Y]
    参阅全部 MEK1 一抗
  • 描述
    兔单克隆抗体[EP2364Y] to MEK1 (phospho T286)
  • 特异性This antibody detects MEK1 phosphorylated at threonine 286.
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho-specific peptide corresponding to residues surrounding threonine 286 of human MEK1

  • 阳性对照
    • A431 cell lysate
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab68140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明IP: 1/50.
    WB: 1/2000. Predicted molecular weight: 43 kDa.

    Is unsuitable for Flow Cyt, ICC or IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
    • 组织特异性Widely expressed, with extremely low levels in brain.
    • 疾病相关Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
      Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Dual specificity mitogen activated protein kinase kinase 1 antibody
      • Dual specificity mitogen-activated protein kinase kinase 1 antibody
      • ERK activator kinase 1 antibody
      • MAP kinase kinase 1 antibody
      • MAP kinase/Erk kinase 1 antibody
      • MAP2K1 antibody
      • MAPK/ERK kinase 1 antibody
      • MAPKK 1 antibody
      • MAPKK1 antibody
      • MEK 1 antibody
      • Mek1 antibody
      • MEKK1 antibody
      • Mitogen activated protein kinase kinase 1 antibody
      • MKK 1 antibody
      • MKK1 antibody
      • MP2K1_HUMAN antibody
      • PRKMK1 antibody
      • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
      • Protein kinase mitogen activated, kinase 1 antibody
      see all

    Anti-MEK1 (phospho T286) antibody [EP2364Y] 图像

    • All lanes : Anti-MEK1 (phospho T286) antibody [EP2364Y] (ab68140) at 1/2000 dilution

      Lane 1 : A431 cell lysate, untreated
      Lane 2 : A431 cell lysate, treated with nocodazole

      Lysates/proteins at 10 µg per lane.

      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 43 kDa
      Observed band size : 43 kDa

    Anti-MEK1 (phospho T286) antibody [EP2364Y] (ab68140)参考文献

    ab68140 has not yet been referenced specifically in any publications.

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