Anti-MEK1 (phospho T286)抗体[EP2364Y] (ab68140)

概述

• 产品名称
  Anti-MEK1 (phospho T286)抗体[EP2364Y]
  参阅全部 MEK1 一抗
• 描述
  兔单克隆抗体[EP2364Y] to MEK1 (phospho T286)
• 特异性
  This antibody detects MEK1 phosphorylated at threonine 286.
• 经测试应用
  适用于: WB, IPmore details
  不适用于: Flow Cyt,ICC or IHC-P
• 种属反应性
  与反应: Human
  
• 免疫原

  A phospho-specific peptide corresponding to residues surrounding threonine 286 of human MEK1

• 阳性对照
  • A431 cell lysate
• 常规说明

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  A trial size is available to purchase for this antibody.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
• 存储溶液
  pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• 纯度
  Tissue culture supernatant
• 克隆
  单克隆
• 克隆编号
  EP2364Y
• 同种型
  IgG
• 研究领域
  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

应用

靶标

• 功能
  Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
• 组织特异性
  Widely expressed, with extremely low levels in brain.
• 疾病相关
  Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
• 序列相似性
  Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
  Contains 1 protein kinase domain.
• 翻译后修饰
  Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
  Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.

• Target information above from: UniProt accession Q02750 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 5604 Human
  • Omim: 176872 Human
  • SwissProt: Q02750 Human
  • Unigene: 145442 Human
• 别名
  • Dual specificity mitogen activated protein kinase kinase 1 antibody
  • Dual specificity mitogen-activated protein kinase kinase 1 antibody
  • ERK activator kinase 1 antibody

  • MAP kinase kinase 1 antibody
  • MAP kinase/Erk kinase 1 antibody
  • MAP2K1 antibody
  • MAPK/ERK kinase 1 antibody
  • MAPKK 1 antibody
  • MAPKK1 antibody
  • MEK 1 antibody
  • Mek1 antibody
  • MEKK1 antibody
  • Mitogen activated protein kinase kinase 1 antibody
  • MKK 1 antibody
  • MKK1 antibody
  • MP2K1_HUMAN antibody
  • PRKMK1 antibody
  • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
  • Protein kinase mitogen activated, kinase 1 antibody

  see all

实验方案

• Western blot protocols
• Immunoprecipitation protocols