重组
RabMAb

Anti-MEK1 (phospho S298)抗体[EPR3338] (ab96379)

概述

  • 产品名称
    Anti-MEK1 (phospho S298)抗体[EPR3338]
    参阅全部 MEK1 一抗
  • 描述
    兔单克隆抗体[EPR3338] to MEK1 (phospho S298)
  • 特异性
    ab96379 detects MEK1 phosphorylated at threonine 298.
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Frmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding serine 298 of Human MEK1.

  • 阳性对照
    • WB: HeLa cell lysates IHC-P: colonic carcinoma tissue ICC/IF: HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab96379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
IHC-Fr 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
    • 组织特异性
      Widely expressed, with extremely low levels in brain.
    • 疾病相关
      Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    • 序列相似性
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰
      Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
      Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Dual specificity mitogen activated protein kinase kinase 1 antibody
      • Dual specificity mitogen-activated protein kinase kinase 1 antibody
      • ERK activator kinase 1 antibody
      • MAP kinase kinase 1 antibody
      • MAP kinase/Erk kinase 1 antibody
      • MAP2K1 antibody
      • MAPK/ERK kinase 1 antibody
      • MAPKK 1 antibody
      • MAPKK1 antibody
      • MEK 1 antibody
      • Mek1 antibody
      • MEKK1 antibody
      • Mitogen activated protein kinase kinase 1 antibody
      • MKK 1 antibody
      • MKK1 antibody
      • MP2K1_HUMAN antibody
      • PRKMK1 antibody
      • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
      • Protein kinase mitogen activated, kinase 1 antibody
      see all

    图片

    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-5-Fluorowillardiine (ab120036), by ICC/IF. Increase in expression of MEK1 (phospho S298) correlates with increased concentration of (S)-5-Fluorowillardiine, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120036 ((S)-5-Fluorowillardiine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
    • ab96379 staining MEK1 (phospho S298) in the NIH3T3  cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-MEK1 (phospho S298) antibody [EPR3338] (ab96379) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : HeLa cell lysates treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP/AP polymerized antibody

      Predicted band size : 43 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    • ab96379, at a 1/100 dilution, staining MEK1 in paraffin embedded Human colonic carcinoma tissue by Immunohistochemical analysis.
    • ab96379, at a 1/100 dilution, staining MEK1 in HeLa cells by Immunofluorescent analysis.
    • ab96379 showing positive staining in Thyroid gland carcinoma tissue.

    • ab96379 showing positive staining in Glioma tissue.

    • ab96379 showing positive staining in Ovarian carcinoma tissue.

    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX (ab120017), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX, as described in literature.
      The cells were incubated at 37øC for 24h in media containing different concentrations of ab120017 (CNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX disodium salt (ab120044), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX disodium salt, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120044 (CNQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX disodium salt (ab120046), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX disodium salt, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120046 (NBQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with DNQX disodium salt (ab120169), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of DNQX disodium salt, as described in literature.
      The cells were incubated at 37°C for 3h in media containing different concentrations of ab120169 (DNQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-5-Chlorowillardiine (ab120062), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-5-Chlorowillardiine, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120062 ((S)-5-Chlorowillardiine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-5-Nitrowillardiine (ab120063), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-5-Nitrowillardiine, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120063 ((S)-5-Nitrowillardiine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (R,S)-AMPA (ab120130), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of(R,S)-AMPA, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120130 ((R,S)-AMPA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-AMPA (ab120005), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-AMPA, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120005 ((S)-AMPA) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with GYKI 52466 (ab120336), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of GYKI 52466, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120336 (GYKI 52466) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX (ab120045), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120045 (NBQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with DNQX (ab120018), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of DNQX, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120018 (DNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
    • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-Williardine (ab120040), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-Williardine, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120040 ((S)-Williardine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

    文献

    This product has been referenced in:
    • Gao Q  et al. Arsenic trioxide inhibits tumor-induced myeloid-derived suppressor cells and enhances T-cell activity. Oncol Lett 13:2141-2150 (2017). WB ; Mouse . Read more (PubMed: 28454374) »
    • Liu J  et al. Lipopolysaccharide Stimulates Surfactant Protein-A in Human Renal Epithelial HK-2 Cells through Upregulating Toll-like Receptor 4 Dependent MEK1/2-ERK1/2-NF-?B Pathway. Chin Med J (Engl) 130:1236-1243 (2017). Read more (PubMed: 28485325) »

    See all 4 Publications for this product

    客户评价及客户问答

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HuH7 and HT29 tumor cells)
    Specification
    HuH7 and HT29 tumor cells
    Blocking step
    Triton-X-100 PBS supplemented with 10% rat serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 0.5%
    Fixative
    Paraformaldehyde
    Username

    Paul Dent

    Verified customer

    提交于 Feb 16 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (NIH3T3)
    Specification
    NIH3T3
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 22 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HUVEK cells)
    Specification
    HUVEK cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 23 2012

    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (COS1 cell line)
    Loading amount
    25 µg
    Specification
    COS1 cell line
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 23 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse embryonic whole lung)
    Loading amount
    25 µg
    Specification
    Mouse embryonic whole lung
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 23 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    25 µg
    Specification
    HEK293
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 23 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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