概述

  • 产品名称Anti-MEK1抗体[Y77]
    参阅全部 MEK1 一抗
  • 描述
    兔单克隆抗体[Y77] to MEK1
  • 特异性The antibody does not crossreact with other MAP kinase kinase family members.
  • 经测试应用适用于: WB, ICC, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to residues in the C terminus of human MEK1.

  • 阳性对照
    • WB: Jurkat cell lysate. IHC: Urinary bladder carcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
ICC 1/250 - 1/500.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

靶标

  • 功能Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
  • 组织特异性Widely expressed, with extremely low levels in brain.
  • 疾病相关Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
    Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dual specificity mitogen activated protein kinase kinase 1 antibody
    • Dual specificity mitogen-activated protein kinase kinase 1 antibody
    • ERK activator kinase 1 antibody
    • MAP kinase kinase 1 antibody
    • MAP kinase/Erk kinase 1 antibody
    • MAP2K1 antibody
    • MAPK/ERK kinase 1 antibody
    • MAPKK 1 antibody
    • MAPKK1 antibody
    • MEK 1 antibody
    • Mek1 antibody
    • MEKK1 antibody
    • Mitogen activated protein kinase kinase 1 antibody
    • MKK 1 antibody
    • MKK1 antibody
    • MP2K1_HUMAN antibody
    • PRKMK1 antibody
    • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
    • Protein kinase mitogen activated, kinase 1 antibody
    see all

Anti-MEK1 antibody [Y77] 图像

  • Anti-MEK1 antibody [Y77] (ab32576) at 1/10000 dilution + Jurkat cell lysate

    Predicted band size : 43 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • Ab32576, at a 1/250 dilution, staining MEK1 in urinary bladder carcinoma by IHC-P.

Anti-MEK1 antibody [Y77] (ab32576)参考文献

This product has been referenced in:
  • Hennessy BT  et al. A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers. Clin Proteomics 6:129-51 (2010). Dot blot ; Human . Read more (PubMed: 21691416) »
  • Astuti P  et al. MAPK pathway activation delays G2/M progression by destabilizing Cdc25B. J Biol Chem 284:33781-8 (2009). WB ; Human . Read more (PubMed: 19801682) »

See all 4 Publications for this product

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