概述

  • 产品名称Anti-MEIS2抗体
    参阅全部 MEIS2 一抗
  • 描述
    兔多克隆抗体to MEIS2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Horse, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human MEIS2.

    (Peptide available as ab73163.)

  • 阳性对照
    • This antibody gave a positive signal in human, mouse and rat brain tissue lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab73164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human MEIS2 peptide (ab73163).

靶标

  • 组织特异性Expressed in various tissues. In hematopoietic tissues, the lymphoid organs express high levels of MEIS2. Also expressed in some regions of the brain, such as the putamen.
  • 序列相似性Belongs to the TALE/MEIS homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homeobox protein Meis2 antibody
    • HsT18361 antibody
    • Meis (mouse) homolog 2 antibody
    • Meis homeobox 2 antibody
    • Meis homolog 2 antibody
    • Meis1 myeloid ecotropic viral integration site 1 homolog 2 (mouse) antibody
    • Meis1 myeloid ecotropic viral integration site 1 homolog 2 antibody
    • Meis1 related gene 1 antibody
    • Meis1 related protein 1 antibody
    • Meis1-related protein 1 antibody
    • MEIS2 antibody
    • MEIS2_HUMAN antibody
    • MGC2820 antibody
    • MRG1 antibody
    • TALE homeobox protein Meis2 antibody
    see all

Anti-MEIS2 antibody 图像

  • All lanes : Anti-MEIS2 antibody (ab73164) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Brain (Mouse) Tissue Lysate - adult normal tissue
    Lane 3 : Brain (Rat) Tissue Lysate - adult normal tissue

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab73164 in 2% Milk, overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ab73164 staining human brain sections in IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed. Blocking of the sample was done with 5 minutes of peroxidase block and 10 minutes of protein block, followed by staining with ab73164 at 1/200 for 45 minutes at 20°C. A HRP conjugated goat anti-rabbit polyclonal antibody was used as the secondary antibody.

Anti-MEIS2 antibody (ab73164)参考文献

This product has been referenced in:
  • Wu YH  et al. miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2. Int J Mol Sci 16:25199-213 (2015). WB ; Human . Read more (PubMed: 26512644) »

See 1 Publication for this product

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The Anti-MEIS2 antibody (ab73164) has been raised against a synthetic peptide taken from within the region from 450- the C-terminal of human MEIS2 (SwissProt reference O14770). The ant...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step (agent) for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Aug 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Ms Brain Ms Heart Ms Spleen Raw264.7)
Loading amount 20 µg
Specification Ms Brain Ms Heart Ms Spleen Raw264.7
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 8-16%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 May 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"