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Synthetic peptide corresponding to Human MEIS1 aa 200-300 (internal sequence) conjugated to keyhole limpet haemocyanin.
Our Abpromise guarantee covers the use of ab19867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|CHIPseq||Use at an assay dependent concentration. PubMed: 22730288|
|ChIP||Use at an assay dependent concentration. PubMed: 20971928|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40, 48 kDa (predicted molecular weight: 43, 50 kDa). Abcam recommends using milk as the blocking agent.|
IHC image of MEIS1 staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19867, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.