Anti-MEGF11抗体(ab108446)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to MEGF11
  • 经测试应用
    适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 827-876 (LMMEELNPYT KISPALGAER HSVGAVTGIM LLLFLIVVLL GLFAWHRRRQ) of Human MEGF11 (NP_115821)

  • 阳性对照
    • Human fetal muscle lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab108446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 111 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • ab108446 staining MEGF11 in zebrafish retina (muller glia cells) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Tris-HCl was used for antigen retrieval. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-MEGF11 antibody (ab108446) at 1 µg/ml + Human fetal muscle lysate at 10 µg

    Predicted band size : 111 kDa
    Gel concentration: 12%

文献

ab108446 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Muller glia cells)
Specification
Retina, Muller glia cells
Permeabilization
Yes - triton X
Fixative
Paraformaldehyde
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提交于 Oct 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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