Anti-MEF2D (phospho S444)抗体(ab59200)


  • 产品名称Anti-MEF2D (phospho S444)抗体
    参阅全部 MEF2D 一抗
  • 描述
    兔多克隆抗体to MEF2D (phospho S444)
  • 特异性Detects endogenous levels of MEF2D only when phosphorylated at serine 444.
  • 经测试应用适用于: IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide (Human) from around the phosphorylation site of serine 444 (PVSPPS).

  • 阳性对照
    • Human lung carcinoma tissue



Our Abpromise guarantee covers the use of ab59200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.


  • 功能Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis.
  • 序列相似性Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • 发展阶段Present in myotubes and also in undifferentiated myoblasts.
  • 结构域The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
  • 翻译后修饰Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
    Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
    Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • 细胞定位Nucleus. Translocated by HDAC4 to nuclear dots.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686I1536 antibody
    • MADS box transcription factor 2 polypeptide D antibody
    • Mef2d antibody
    • MEF2D_HUMAN antibody
    • Myocyte enhancer factor 2D antibody
    • Myocyte specific enhancer factor 2, polypeptide D antibody
    • Myocyte specific enhancer factor 2D antibody
    • Myocyte-specific enhancer factor 2D antibody
    see all

Anti-MEF2D (phospho S444) antibody 图像

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab59200 at 1/50 dilution. Samples were untreated (left) or treated with immunizing peptide (right).

Anti-MEF2D (phospho S444) antibody (ab59200)参考文献

ab59200 has not yet been referenced specifically in any publications.

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Thank you for your reply.
Mouse is actually also untested for ab59200 - it is listed as predicted to react based on sequence homology. Thus, we would not guarantee it for this species. But, please feel free to submit another Abreview for mouse sam...

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Thank you for submitting an Abreview for ab59200. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested species and application, we would like to follow up on t...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Spermophilus tridecemlineatus Tissue lysate - whole (Heart)
Loading amount 40 µg
Specification Heart
Gel Running Conditions Reduced Denaturing (10% Tris-Glycine)
Blocking step BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mr. James Bjork

Verified customer

提交于 Nov 06 2012

Thank you very much for contacting us with your enquiry. I've created the discount code below-
Expiration date: January 28, 2013
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