概述

  • 产品名称Anti-MEF2D抗体
    参阅全部 MEF2D 一抗
  • 描述
    小鼠单克隆抗体to MEF2D
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human MEF2D protein with tag

  • 阳性对照
    • K-562 cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab60717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

靶标

  • 功能Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis.
  • 序列相似性Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • 发展阶段Present in myotubes and also in undifferentiated myoblasts.
  • 结构域The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
  • 翻译后修饰Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
    Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
    Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • 细胞定位Nucleus. Translocated by HDAC4 to nuclear dots.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686I1536 antibody
    • MADS box transcription factor 2 polypeptide D antibody
    • Mef2d antibody
    • MEF2D_HUMAN antibody
    • Myocyte enhancer factor 2D antibody
    • Myocyte specific enhancer factor 2, polypeptide D antibody
    • Myocyte specific enhancer factor 2D antibody
    • Myocyte-specific enhancer factor 2D antibody
    see all

Anti-MEF2D antibody 图像

  • Anti-MEF2D antibody (ab60717) at 1 µg/ml + K-562 cell lysate at 25 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa

Anti-MEF2D antibody (ab60717)参考文献

This product has been referenced in:
  • Ye J  et al. Translation of Myocyte Enhancer Factor-2 is induced by hypertrophic stimuli in cardiomyocytes through a Calcineurin-dependent pathway. J Mol Cell Cardiol 53:578-87 (2012). Read more (PubMed: 22850285) »

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