Anti-MEF2C抗体[OTI4B10] (ab118406)
Key features and details
- Mouse monoclonal [OTI4B10] to MEF2C
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
-
产品名称
Anti-MEF2C抗体[OTI4B10]
参阅全部 MEF2C 一抗 -
描述
小鼠单克隆抗体[OTI4B10] to MEF2C -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human MEF2C.
-
阳性对照
- WB:HEK293T cell lysate transfected with pCMV6-ENTRY MEF2C cDNA; ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY MEF2C; Flow Cyt (Intra): HeLa and Jurkat cells.
-
常规说明
The clone number has been updated from 4B10 to OTI4B10, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS -
Concentration information loading...
-
纯度
Protein G purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography -
克隆
单克隆 -
克隆编号
OTI4B10 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118406于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500. Predicted molecular weight: 51 kDa.
|
|
ICC/IF |
1/100.
|
|
Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
---|
WB
1/500. Predicted molecular weight: 51 kDa. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2. -
组织特异性
Expressed in brain and skeletal muscle. -
疾病相关
Defects in MEF2C are the cause of mental retardation-stereotypic movements-epilepsy and/or cerebral malformations (MRSME) [MIM:613443]. It is a disorder characterized by severe mental retardation, absent speech, hypotonia, poor eye contact and stereotypic movements. Dysmorphic features include high broad forehead with variable small chin, short nose with anteverted nares, large open mouth, upslanted palpebral fissures and prominent eyebrows. Some patients have seizures. -
序列相似性
Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain. -
发展阶段
Expression is highest during the early stages of postnatal development, at later stages levels greatly decrease. -
结构域
The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity. -
翻译后修饰
Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.
Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation.
Sumoylated on Lys-391 by SUMO2 but not by SUMO1 represses transcriptional activity.
Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 4208 Human
- Omim: 600662 Human
- SwissProt: Q06413 Human
- Unigene: 649965 Human
-
别名
- C5DELq14.3 antibody
- DEL5q14.3 antibody
- MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody
see all
图片
-
All lanes : Anti-MEF2C antibody [OTI4B10] (ab118406) at 1/500 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY MEF2C cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 51 kDa -
ab118406 at 1/100 dilution staining MEF2C in COS7 cells transiently transfected by pCMV6-ENTRY MEF2C by Immunofluorescence.
-
ab118406 at 1/100 dilution staining MEF2C in HeLa cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
-
ab118406 at 1/100 dilution staining MEF2C in Jurkat cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab118406 被引用在 1 文献中.
- McGirt LY et al. miR-223 Regulates Cell Growth and Targets Proto-Oncogenes in Mycosis Fungoides/Cutaneous T-Cell Lymphoma. J Invest Dermatol N/A:N/A (2013). Human . PubMed: 24304814