概述

  • 产品名称Anti-MEF2A抗体[EP1706Y]
    参阅全部 MEF2A 一抗
  • 描述
    兔单克隆抗体[EP1706Y] to MEF2A
  • 经测试应用适用于: WB, ICC, Flow Cyt, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MEF2A aa 400-500.

  • 阳性对照
    • WB: Fetal brain, human heart and HeLa lysates. IHC-P: Human kidney tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab76063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/20 - 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation.
    • 组织特异性Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.
    • 疾病相关Defects in MEF2A might be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320].
    • 序列相似性Belongs to the MEF2 family.
      Contains 1 MADS-box domain.
      Contains 1 Mef2-type DNA-binding domain.
    • 翻译后修饰Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.
      Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated by SUMO1 and, to a lesser extent by SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis.
      Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.
      Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ADCAD1 antibody
      • MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody
      • MEF2 antibody
      • MEF2A antibody
      • MEF2A_HUMAN antibody
      • Myocyte enhancer factor 2A antibody
      • Myocyte-specific enhancer factor 2A antibody
      • RSRFC4 antibody
      • RSRFC9 antibody
      • Serum response factor like protein 1 antibody
      • Serum response factor-like protein 1 antibody
      see all

    Anti-MEF2A antibody [EP1706Y] 图像

    • All lanes : Anti-MEF2A antibody [EP1706Y] (ab76063) at 1/1000 dilution

      Lane 1 : Murine cardiomyocyte whole cell lysate
      Lane 2 : Murine cardiomyocyte whole cell lysate
      Lane 3 : Murine cardiomyocyte whole cell lysate
      Lane 4 : Murine cardiomyocyte whole cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-rabbit polyclonal HRP conjugate.

      Performed under reducing conditions.

      Predicted band size : 55 kDa


      Exposure time : 30 seconds

      Image is courtesy of an anonymous AbReview.

      Lanes from left to right: cropped MW marker ladder, 1, 2, 3, 4.

      Blocking was performed with 5% milk for 1 hour at room temperature.

      See Abreview

    • All lanes : Anti-MEF2A antibody [EP1706Y] (ab76063) at 1/10000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Human heart lysate
      Lane 3 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 55 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
      The observed band size may be different from the predicted molecular weight as the protein is sumoylated.
    • Immunohistochemical analysis of MEF2A in paraffin embedded human kidney tissue using ab76063 at a 1/50 dilution.
    • Overlay histogram showing MCF-7 cells stained with ab76063 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76063, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    Anti-MEF2A antibody [EP1706Y] (ab76063)参考文献

    This product has been referenced in:
    • Malek MH  et al. Similar skeletal muscle angiogenic and mitochondrial signalling following 8 weeks of endurance exercise in mice: discontinuous versus continuous training. Exp Physiol 98:807-18 (2013). Read more (PubMed: 23180811) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Heart)
    Gel Running Conditions Reduced Denaturing (15% PAGE)
    Loading amount 25 µg
    Specification Heart
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 05 2015

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Cardiomyocytes)
    Gel Running Conditions Reduced Denaturing
    Loading amount 25 µg
    Specification Cardiomyocytes
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 28 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"