概述

  • 产品名称Anti-MEF2A抗体
    参阅全部 MEF2A 一抗
  • 描述
    兔多克隆抗体to MEF2A
  • 特异性ab51153 recognises endogenous levels of total MEF2A protein. It can cross-react with isoform MEFA, RSRFC4, RSRFC9, and isoform 5.
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from human MEF2A around the phosphorylation site of serine 408 (P-I-S-P-P).

  • 阳性对照
    • human breast carcinoma, extracts from Hela cells treated with PMA

性能

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应用

Our Abpromise guarantee covers the use of ab51153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation.
  • 组织特异性Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.
  • 疾病相关Defects in MEF2A might be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320].
  • 序列相似性Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • 翻译后修饰Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.
    Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated by SUMO1 and, to a lesser extent by SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis.
    Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADCAD1 antibody
    • MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody
    • MEF2 antibody
    • MEF2A antibody
    • MEF2A_HUMAN antibody
    • Myocyte enhancer factor 2A antibody
    • Myocyte-specific enhancer factor 2A antibody
    • RSRFC4 antibody
    • RSRFC9 antibody
    • Serum response factor like protein 1 antibody
    • Serum response factor-like protein 1 antibody
    see all

Anti-MEF2A antibody 图像

  • All lanes : Anti-MEF2A antibody (ab51153) at 1/500 dilution

    Lane 1 : extracts from Hela cells treated with 125ng/ml PMA for 30min, without immunizing peptide
    Lane 2 : extracts from Hela cells treated with 125ng/ml PMA for 30min, with immunizing peptide


    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Imunohistochemical analysis of MEF2A expression in paraffin-embedded human breast carcinoma tissue sections using 1/50 ab51153. Left: Untreated sample. Right: sample treated with immunising peptide (negative control).
  • ICC/IF image of ab51153 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MEF2A antibody (ab51153)参考文献

This product has been referenced in:
  • Elmer BM  et al. MHCI requires MEF2 transcription factors to negatively regulate synapse density during development and in disease. J Neurosci 33:13791-804 (2013). Read more (PubMed: 23966700) »

See 1 Publication for this product

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