Anti-MEF2A抗体[1A10] (ab117555)

概述

  • 产品名称Anti-MEF2A抗体[1A10]
    参阅全部 MEF2A 一抗
  • 描述
    小鼠单克隆抗体[1A10] to MEF2A
  • 经测试应用适用于: WB, Sandwich ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Human MEF2A protein

  • 阳性对照
    • Human lung cancer lysate; Human Prostate tissue; HeLa cells

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab117555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
Sandwich ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation.
  • 组织特异性Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.
  • 疾病相关Defects in MEF2A might be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320].
  • 序列相似性Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • 翻译后修饰Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.
    Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated by SUMO1 and, to a lesser extent by SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis.
    Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADCAD1 antibody
    • MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody
    • MEF2 antibody
    • MEF2A antibody
    • MEF2A_HUMAN antibody
    • Myocyte enhancer factor 2A antibody
    • Myocyte-specific enhancer factor 2A antibody
    • RSRFC4 antibody
    • RSRFC9 antibody
    • Serum response factor like protein 1 antibody
    • Serum response factor-like protein 1 antibody
    see all

Anti-MEF2A antibody [1A10] 图像

  • Anti-MEF2A antibody [1A10] (ab117555) at 1/500 dilution + Human lung cancer lysate

    Predicted band size : 55 kDa
  • ab117555, at 2.5µg/ml, staining MEF2A in formalin-fixed, paraffin-embedded Human Prostate by Immunohistochemistry.
  • ab117555, at 10 µg/ml, staining MEF2A in Hela cells by Immunofluorescence.
  • Detection limit for recombinant tagged MEF2A is approximately 0.3ng/ml as a capture antibody.

Anti-MEF2A antibody [1A10] (ab117555)参考文献

ab117555 has not yet been referenced specifically in any publications.

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