Full length human MED9 protein (NP_060489)
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein G purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa).
MED9 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes.
- FLJ10193 antibody
- MED25 antibody
- MED9 antibody
Western blot - Anti-MED9 antibody (ab69323)
All lanes :
Anti-MED9 antibody (ab69323) at 1/500 dilutionLane 1 :
MED9 transfected 293T cell lysateLane 2 :
Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.SecondaryAll lanes :
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionPredicted band size:
16 kDaObserved band size:
20 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"