概述

  • 产品名称Anti-MED8抗体
    参阅全部 MED8 一抗
  • 描述
    兔多克隆抗体to MED8
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (QREEKQLEAS LDALLSQVAD LKNSLGSFIC KLENEYGRLT WPSVLDSFAL) of Human MED8 (NP_443109).

  • 阳性对照
    • Fetal stomach lysate.

性能

应用

Our Abpromise guarantee covers the use of ab87222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the Mediator complex subunit 8 family.
  • 结构域The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activator recruited cofactor 32 kDa component antibody
    • Activator-recruited cofactor 32 kDa component antibody
    • ARC32 antibody
    • med8 antibody
    • MED8_HUMAN antibody
    • Mediator complex subunit 8 antibody
    • Mediator of RNA polymerase II transcription subunit 8 antibody
    • Mediator of RNA polymerase II transcription subunit 8 homolog antibody
    see all

Anti-MED8 antibody 图像

  • Anti-MED8 antibody (ab87222) at 1 µg/ml (5% skim milk / PBS buffer) + fetal stomach lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)

Anti-MED8 antibody (ab87222)参考文献

ab87222 has not yet been referenced specifically in any publications.

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