Anti-MED6抗体- ChIP Grade (ab84968)

概述

  • 产品名称Anti-MED6抗体- ChIP Grade
    参阅全部 MED6 一抗
  • 描述
    兔多克隆抗体to MED6 - ChIP Grade
  • 经测试应用适用于: WB, ChIPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide within residues: LLLDLRQKFP PKFVQLKPGE KPVPVDQTKK EAEPIPETVK PEEKETTKNV, corresponding to internal sequence amino acids 180-229 of Human MED6 (NP_005457)

  • 阳性对照
    • 721_B cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use at an assay dependent concentration.

靶标

  • 功能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 序列相似性Belongs to the Mediator complex subunit 6 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activator recruited cofactor 33 kDa antibody
    • Activator-recruited cofactor 33 kDa component antibody
    • ARC33 antibody
    • hMed6 antibody
    • MED6 antibody
    • MED6_HUMAN antibody
    • Mediator complex subunit 6 antibody
    • Mediator of RNA polymerase II transcription subunit 6 antibody
    • NY REN 28 antigen antibody
    • Renal carcinoma antigen NY-REN-28 antibody
    • RNA polymerase transcriptional regulation mediator subunit 6 antibody
    see all

Anti-MED6 antibody - ChIP Grade 图像

  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

  • Anti-MED6 antibody - ChIP Grade (ab84968) at 1 µg/ml + 721_B cell lysate at 10 µg/ml

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

Anti-MED6 antibody - ChIP Grade (ab84968)参考文献

ab84968 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab84968.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"