Anti-MED31抗体- ChIP Grade (ab98142)

概述

  • 产品名称Anti-MED31抗体- ChIP Grade
    参阅全部 MED31 一抗
  • 描述
    兔多克隆抗体to MED31 - ChIP Grade
  • 经测试应用适用于: WB, ChIPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N-terminal amino acids 1-50 (MAAAVAMETDDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFV N) of Human MED31 (NP_057144).

  • 阳性对照
    • Human fetal liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab98142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use at an assay dependent concentration.

靶标

  • 相关性MED31 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • SOH1 antibody
    • CGI-125 antibody
    • hSOH1 antibody
    • Mediator complex subunit 31 antibody
    • Mediator complex subunit SOH1 antibody
    • Mediator of RNA polymerase II transcription subunit 31 antibody
    see all

Anti-MED31 antibody - ChIP Grade 图像

  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

  • Anti-MED31 antibody - ChIP Grade (ab98142) at 1 µg/ml (5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50,000

    Predicted band size : 16 kDa
    Gel concentration: 10-20%

Anti-MED31 antibody - ChIP Grade (ab98142)参考文献

ab98142 has not yet been referenced specifically in any publications.

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