Anti-MED25抗体(ab107551)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to MED25
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to N terminal amino acids 119-148 of Human MED25 (NP_112235.2).

  • 阳性对照
    • Mouse kidney tissue lysates; MCF7 cell line lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab107551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 78 kDa.

靶标

  • 功能
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.
  • 组织特异性
    Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen.
  • 疾病相关
    Defects in MED25 are the cause of Charcot-Marie-Tooth disease type 2B2 (CMT2B2) [MIM:605589]. It is a recessive axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies(designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced.
  • 序列相似性
    Belongs to the Mediator complex subunit 25 family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACID1 antibody
    • Activator interaction domain 1 protein antibody
    • Activator interaction domain-containing protein 1 antibody
    • Activator-recruited cofactor 92 kDa component antibody
    • ARC/mediator transcriptional coactivator subunit antibody
    • ARC/mediator transcriptional coactivator subunit ARC92 antibody
    • ARC92 antibody
    • BVSYS antibody
    • CMT2B2 antibody
    • DKFZp434K0512 antibody
    • FLJ10193 antibody
    • med25 antibody
    • MED25 protein antibody
    • MED25_HUMAN antibody
    • Mediator complex subunit 25 antibody
    • Mediator of RNA polymerase II transcription subunit 25 antibody
    • Mediator of RNA polymerase II transcription, subunit 25 homolog (S cerevisiae) antibody
    • Mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) antibody
    • MGC70671 antibody
    • p78 antibody
    • prostate-derived protein 78 kDa antibody
    • PTOV2 antibody
    • TCBAP0758 antibody
    see all

Anti-MED25 antibody 图像

  • Anti-MED25 antibody (ab107551) at 1/100 dilution + Mouse kidney tissue lysates at 35 µg

    Predicted band size : 78 kDa
  • Anti-MED25 antibody (ab107551) at 1/100 dilution + MCF7 cell line lysates at 35 µg

    Predicted band size : 78 kDa

实验方案

Anti-MED25 antibody (ab107551)参考文献

ab107551 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - nuclear (Mouse embryonic Stem Cells)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
35 µg
Specification
Mouse embryonic Stem Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Jan-Aurel Pfister

Verified customer

提交于 Apr 10 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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