这个 快线抗体 还未被充份测试和鉴定,因此它受制于相关的 使用条款

概述

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

  • 应用说明
    IF/ICC: see image below

    This antibody gave a positive result in ELISA against the immunizing peptide (ab58444).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
    • 序列相似性
      Belongs to the Mediator complex subunit 19 family.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • LCMR1 antibody
      • Lung cancer metastasis related protein 1 antibody
      • Lung cancer metastasis-related protein 1 antibody
      • med19 antibody
      • MED19_HUMAN antibody
      • Mediator complex subunit 19 antibody
      • Mediator of RNA polymerase II transcription subunit 19 antibody
      see all

    图片

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • ICC/IF image of ab49271 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49271, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2 cells fixed in 4% PFA at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
    • All lanes : Anti-MED19 antibody (ab49271) at 1 µg

      Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Predicted band size : 20 kDa
      Observed band size : 20 kDa
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    文献

    ab49271 has not yet been referenced specifically in any publications.

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