概述

  • 产品名称Anti-MED13L抗体
    参阅全部 MED13L 一抗
  • 描述
    兔多克隆抗体to MED13L
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
  • 免疫原

    Synthetic peptide within Human MED13L aa 550-600. The exact sequence is proprietary. (GeneID 23389).
    Database link: NP_056150.1

  • 阳性对照
    • Whole cell lysate from HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab87831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
  • 组织特异性Highly expressed in brain (cerebellum), heart (aorta), skeletal muscle, kidney, placenta and peripheral blood leukocytes. Highly expressed in fetal brain.
  • 疾病相关Defects in MED13L are a cause of transposition of the great arteries, dextro-looped (DTGA) [MIM:608808]. DTGA consists of complete inversion of the great vessels, so that the aorta incorrectly arises from the right ventricle and the pulmonary artery incorrectly arises from the left ventricle. This creates completely separate pulmonary and systemic circulatory systems, an arrangement that is incompatible with life. Patients often have atrial and/or ventricular septal defects or other types of shunting that allow some mixing between the circulations in order to support life minimally, but surgical intervention is always required.
    Note=A chromosomal aberration involving MED13L is found in a patient with transposition of the great arteries, dextro-looped and mental retardation. Translocation t(12;17)(q24.1;q21).
  • 序列相似性Belongs to the Mediator complex subunit 13 family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MD13L_HUMAN antibody
    • MED13L antibody
    • Mediator complex subunit 13 like antibody
    • Mediator complex subunit 13-like antibody
    • Mediator of RNA polymerase II transcription subunit 13-like antibody
    • THRAP2 antibody
    • Thyroid hormone receptor-associated protein 2 antibody
    • Thyroid hormone receptor-associated protein complex 240 kDa component-like antibody
    • TRAP240L antibody
    see all

Anti-MED13L antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED13L with ab87831 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MED13L antibody (ab87831) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg/ml
    Lane 2 : HeLa whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 5 µg/ml

    Developed using the ECL technique

    Predicted band size : 243 kDa


    Exposure time : 3 minutes
  • Detection of MED13L by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87831 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection. Chemiluminescence with an exposure time of 30 seconds.

Anti-MED13L antibody (ab87831)参考文献

This product has been referenced in:
  • Tsai KL  et al. A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction. Nat Struct Mol Biol 20:611-9 (2013). WB ; Human . Read more (PubMed: 23563140) »

See 1 Publication for this product

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