这个 快线抗体 还未被充份测试和鉴定,因此它受制于相关的 使用条款

Anti-MED13抗体(ab49468)

概述

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

  • 应用说明
    This antibody gave a positive result in ELISA against the immunizing peptide (ab60790).
    IF/ICC: see figure below
    Not yet tested in other applications.

  • 靶标

    • 相关性
      MED13 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
    • 细胞定位
      Nuclear
    • 数据库链接
    • 别名
      • 1110067M05Rik antibody
      • Activator-recruited cofactor 250 kDa component antibody
      • ARC250 antibody
      • D030023K18 antibody
      • DRIP 250 antibody
      • DRIP250 antibody
      • HSPC221 antibody
      • KIAA0593 antibody
      • Mediator complex subunit 13 antibody
      • Mediator of RNA polymerase II transcription subunit 13 homolog antibody
      • Mediator of RNA polymerase II transcription, subunit 13 antibody
      • OTTMUSP00000001175 antibody
      • RP23-50E4.3 antibody
      • THRAP1 antibody
      • Thyroid hormone receptor associated protein 240 kDa subunit antibody
      • Thyroid hormone receptor associated protein complex component TRAP240 antibody
      • Thyroid hormone receptor-associated protein 1 antibody
      • Thyroid hormone receptor-associated protein complex 240 kDa component antibody
      • Trap240 antibody
      • Vitamin D3 receptor-interacting protein complex component DRIP250 antibody
      see all

    图片

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • ICC/IF image of ab49468 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49468, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek, Hep and MCF7 cells fixed in PFA and HeLa, Hek293, HepG2 and MCF7 fixed in 100% methanol, all at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

    文献

    ab49468 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Yes you are correct. BSA, goat serum and glycine are diluted in PBS. I hope this helps. Please let me know if you need anything further.

    Thank you for your enquiry. ab49468 is considered a "Fast track" antibody. Fast track antibodies have only minimal characterization and as such are offered at a reduced price compared to our typical primary antibodies. ab49468 has been tested...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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