Anti-MED10抗体(ab110786)

概述

  • 产品名称
    Anti-MED10抗体
  • 描述
    兔多克隆抗体to MED10
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MED10.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293; HeLa; Jurkat; MCF7; Caco2.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab110786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.

Use with paraformaldehyde fixed cells.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 16 kDa).

靶标

  • 功能
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 序列相似性
    Belongs to the Mediator complex subunit 10 family.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • L6 antibody
    • med10 antibody
    • MED10_HUMAN antibody
    • Mediator complex subunit 10 antibody
    • Mediator of RNA polymerase II transcription subunit 10 antibody
    • Mediator of RNA polymerase II transcription, subunit 10 homolog antibody
    • NUT2 antibody
    • Transformation-related gene 17 protein antibody
    • Transformation-related gene 20 protein antibody
    • Transformation-related protein 17 antibody
    • Transformation-related protein 20 antibody
    • TRG-17 antibody
    • TRG-20 antibody
    • TRG17 antibody
    • TRG20 antibody
    see all

图片

  • ab110786 (1/200) staining MED10 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details, please see Abreview.

    See Abreview

  • All lanes : Anti-MED10 antibody (ab110786) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,21 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab110786 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

文献

ab110786 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 May 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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