概述

  • 产品名称
    Anti-MeCP2抗体[Mec-168]
    参阅全部 MeCP2 一抗
  • 描述
    小鼠单克隆抗体[Mec-168] to MeCP2
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to Human MeCP2 aa 471-486 (C terminal).
    Sequence:

    REEPVDSRTPVTERVS


    Database link: P51608

  • 阳性对照
    • Jurkat cell nuclear extract, human acute T cell leukemia cells.
  • 常规说明


    Antibodies reacting specifically with MeCP2 may be used for studying chromatin remodeling effects on gene expression.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    IgG fraction
  • Primary antibody说明
    Antibodies reacting specifically with MeCP2 may be used for studying chromatin remodeling effects on gene expression.
  • 克隆
    单克隆
  • 克隆编号
    Mec-168
  • 骨髓瘤
    NS1
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 52 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A.
  • 组织特异性
    Present in all adult somatic tissues tested.
  • 疾病相关
    Defects in MECP2 may be a cause of Angelman syndrome (AS) [MIM:105830]; also known as happy puppet syndrome. AS is a neurodevelopmental disorder characterized by severe mental retardation, absent speech, ataxia, sociable affect and dysmorphic facial features. AS and Rett syndrome have overlapping clinical features.
    Defects in MECP2 are the cause of mental retardation syndromic X-linked type 13 (MRXS13) [MIM:300055]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXS13 patients manifest mental retardation associated with other variable features such as spasticity, episodes of manic depressive psychosis, increased tone and macroorchidism.
    Defects in MECP2 are the cause of Rett syndrome (RTT) [MIM:312750]. RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Patients appear to develop normally until 6 to 18 months of age, then gradually lose speech and purposeful hand movements and develop microcephaly, seizures, autism, ataxia, intermittent hyperventilation, and stereotypic hand movements. After initial regression, the condition stabilizes and patients usually survive into adulthood.
    Defects in MECP2 may be the cause of susceptibility autism X-linked type 3 (AUTSX3) [MIM:300496]. AUTSX3 is a pervasive developmental disorder (PDD), prototypically characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age.
    Defects in MECP2 are the cause of encephalopathy neonatal severe due to MECP2 mutations (ENS-MECP2) [MIM:300673]. Note=The MECP2 gene is mutated in Rett syndrome, a severe neurodevelopmental disorder that almost always occurs in females. Although it was first thought that MECP2 mutations causing Rett syndrome were lethal in males, later reports identified a severe neonatal encephalopathy in surviving male sibs of patients with Rett syndrome. Additional reports have confirmed a severe phenotype in males with Rett syndrome-associated MECP2 mutations.
    Defects in MECP2 are the cause of mental retardation syndromic X-linked Lubs type (MRXSL) [MIM:300260]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSL patients manifest mental retardation associated with variable features. They include swallowing dysfunction and gastroesophageal reflux with secondary recurrent respiratory infections, hypotonia, mild myopathy and characteristic facies such as downslanting palpebral fissures, hypertelorism and a short nose with a low nasal bridge. Note=Increased dosage of MECP2 due to gene duplication appears to be responsible for the mental retardation phenotype.
  • 序列相似性
    Contains 2 A.T hook DNA-binding domains.
    Contains 1 MBD (methyl-CpG-binding) domain.
  • 翻译后修饰
    Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.
  • 细胞定位
    Nucleus. Colocalized with methyl-CpG in the genome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AUTSX 3 antibody
    • AUTSX3 antibody
    • DKFZp686A24160 antibody
    • Mbd 5 antibody
    • Mbd5 antibody
    • MECP 2 antibody
    • MeCP 2 protein antibody
    • MeCP-2 protein antibody
    • Mecp2 antibody
    • MECP2_HUMAN antibody
    • Methyl CpG binding protein 2 (Rett syndrome) antibody
    • Methyl CpG binding protein 2 antibody
    • Methyl-CpG-binding protein 2 antibody
    • MRX 16 antibody
    • MRX 79 antibody
    • MRX16 antibody
    • MRX79 antibody
    • MRXS 13 antibody
    • MRXS13 antibody
    • MRXSL antibody
    • PPMX antibody
    • RS antibody
    • RTS antibody
    • RTT antibody
    • WBP 10 antibody
    • WBP10 antibody
    see all

图片

  • ab50005 staining MeCP2 in mouse E15.5 embryo/eye analge by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with modified citrate buffer pH6.1 and blocked for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. An Cy3®-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-MeCP2 antibody [Mec-168] (ab50005) at 0.5 µg/ml + Nuclear extract from Jurkat cells

    Predicted band size : 52 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence studies of NB54-treated (D–F) R294X fibroblasts, using MeCP2 C-terminal antibody ab50005. Staining of the full-length MeCP2 (green signal in D, F) corresponds with the DAPI staining (blue signal in E, F), thus indicating its nuclear localization.

    Cells were fixed with 4% paraformaldehyde in PBS for 20 min. Permeabilization included incubation with 0.1% Triton X-100 for 5 min and blocking with 5% skimmed milk in TBS containing 0.1% Tween 20 for 30 minutes at room temperature. Incubations with primary antibody ab50005 (1/1000) and secondary antibody Alexa Fluor® 488-conjugated goat anti-mouse (1/500) were performed in blocking solution for 1 hour

文献

This product has been referenced in:

See all 15 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Rodent Block (Zytomed) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Sample
Mouse Cell (E15.5 embryo/ eye anlage)
Specification
E15.5 embryo/ eye anlage
Permeabilization
Yes - Modified citrate buffer pH6.1
Fixative
Paraformaldehyde
Username

Mr. Eike Wegener

Verified customer

提交于 Apr 15 2014

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