人Anti-Measles virus IgG ELISA试剂盒(ab108750)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
概述
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产品名称
人Anti-Measles virus IgG ELISA试剂盒
参阅全部 Measles Virus IgG 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Pos. Serum 18 9.4% Pos. Serum 24 3.2% 批次间 样品 n Mean SD CV% Pos. Serum 12 5% Pos. Serum 12 1.5% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Indirect -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s anti-Measles virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Measles virus in Human serum and plasma.
A 96-well plate has been precoated with Measles virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Measles virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Measles virus IgG sample captured in plate.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Measles IgG Cut-off Control Yelow, Green cap 1 x 3ml Measles IgG Negative Control Yellow, Blue cap 1 x 2ml Measles IgG Positive Control Yellow, Red cap 1 x 2ml Measles virus (IgG) Coated Microplate (12 x 8 wells) 1 unit Measles virus anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究领域
相关产品
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Related Products
图片
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab108750 被引用在 2 文献中.
- Prahl M et al. Exposure to pesticides in utero impacts the fetal immune system and response to vaccination in infancy. Nat Commun 12:132 (2021). PubMed: 33420104
- Ciencewicki JM et al. Plasma Donors in the Southwestern United States Positively Contribute to the Diverse Therapeutic Antibody Profile of Immune Globulin Products. Sci Rep 10:6850 (2020). PubMed: 32321978