Anti-Measles nucleoprotein抗体[2D7] (ab9882)

概述

  • 产品名称
    Anti-Measles nucleoprotein抗体[2D7]
    参阅全部 Measles nucleoprotein 一抗
  • 描述
    小鼠单克隆抗体[2D7] to Measles nucleoprotein
  • 特异性
    Reacts with measles NP. Does not cross-react with mumps NP. Recognises both native and recombinant yeast-derived measles NP.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 免疫原

    Recombinant measles NP

  • 阳性对照
    • recombinant measles NP
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    PBS with 0.1% sodium azide
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
    单克隆
  • 克隆编号
    2D7
  • 骨髓瘤
    NS0/1
  • 同种型
    IgG1
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000 - 1/10000.
WB 1/500 - 1/2000. Detects a band of approximately 52.5 kDa.

靶标

  • 相关性
    Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest
  • 细胞定位
    Virion. Host cytoplasm.
  • 别名
    • Measles nucleocapsid protein antibody
    • N antibody
    • NP antibody
    • Nucleocapsid protein antibody

Anti-Measles nucleoprotein antibody [2D7] 图像

  • Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
    Lane 1 - prestained marker proteins
    Lane 2 - recombinant measles NP.

实验方案

Anti-Measles nucleoprotein antibody [2D7] (ab9882)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for your enquiry. The molecular weight of the protein (measles nucleocapsid protein) is 52.5 kDa. The size of the orange marker protein is 70 kDa. Please contact us again if you have additional questions.

Thank you for your enquiry about our Measles nucleoprotein antibodies. A nucleoprotein is defined as any of a group of complexes composed of protein and nucleic acid and found in the nuclei and cytoplasm of all living cells, as in chromatin and rib...

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Thank you for the information that you have provided and I'm sorry to hear that your customer is experiencing difficulty with ab9882. We don't have lot 200502 in stock anymore. This is the first complaint that we have received concerning this antibody ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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