antigen sequence: ALNNHSSIPM DPEHVELVKR TMAGVSLPAP GVPAWAREIS DAQWEDVVQK ALQARQAS, corresponding to internal sequence amino acids 124-181 of Human MEA1.
- Human testis tissue; MEA1 over-expression lysate from HEK293 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
May play an important role in spermatogenesis and/or testis development.
Highly expressed in testis.
Information by UniProt
- HYS antibody
- Male enhanced antigen (H Y structural gene) antibody
- Male-enhanced antigen 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-MEA1 antibody (ab121543)
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121543 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEA1 antibody (ab121543)
ab121543 at 1/20 dilution staining MEA1 in paraffin-embedded Human testis tissue by Immunohistochemistry
Western blot - Anti-MEA1 antibody (ab121543)
All lanes : Anti-MEA1 antibody (ab121543) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : MEA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
has not yet been referenced specifically in any publications.
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