概述

  • 产品名称Anti-ME3抗体
    参阅全部 ME3 一抗
  • 描述
    兔多克隆抗体to ME3
  • 特异性This antibody detects endogenous levels of total ME3 protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthesized peptide derived from an internal region of human ME3.

  • 阳性对照
    • Extracts from 3T3 cells and RAW264.7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab74258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 67 kDa.
ELISA 1/40000.

靶标

  • 相关性Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+) dependent isoform, and a mitochondrial NAD(+) dependent isoform. This gene encodes a mitochondrial NADP(+) dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. ME3 is expressed predominantly in organs with a low division rate. Its catalytic activity is (S) malate + NADP+ = pyruvate + CO2 + NADPH.
  • 细胞定位Mitochondrion matrix.
  • 数据库链接
  • 别名
    • Malate dehydrogenase antibody
    • Malic enzyme 3 antibody
    • Malic enzyme 3, NADP(+) dependent, mitochondrial antibody
    • NADP ME antibody
    • NADPME antibody
    • Pyruvic malic carboxylase antibody
    see all

Anti-ME3 antibody 图像

  • All lanes : Anti-ME3 antibody (ab74258) at 1/500 dilution

    Lane 1 : Extracts from 3T3 cells
    Lane 2 : Extracts from RAW264.7 cells
    Lane 3 : Extracts from RAW264.7 cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 67 kDa
    Observed band size : 67 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

Anti-ME3 antibody (ab74258)参考文献

ab74258 has not yet been referenced specifically in any publications.

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