概述

  • 产品名称Anti-ME1抗体
    参阅全部 ME1 一抗
  • 描述
    小鼠单克隆抗体to ME1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human ME1 protein (AAH25246) with a proprietary tag.

  • 阳性对照
    • WB: HepG2 and ME1 transfected 293T cell lysates IHC-P: human tonsil tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab87974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 64 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 µg/ml.

靶标

  • 组织特异性Expressed in all tissues tested including liver, placenta and white adipose tissue.
  • 序列相似性Belongs to the malic enzymes family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytosolic malic enzyme 1 antibody
    • HUMNDME antibody
    • Malate dehydrogenase antibody
    • Malic enzyme 1 antibody
    • Malic enzyme 1, NADP(+) dependent, cytosolic antibody
    • Malic enzyme 1, soluble antibody
    • Malic enzyme, cytoplasmic antibody
    • MAOX_HUMAN antibody
    • ME1 antibody
    • MES antibody
    • NADP dependent malic enzyme antibody
    • NADP ME antibody
    • NADP-dependent malic enzyme antibody
    • NADP-ME antibody
    • Pyruvic malic carboxylase antibody
    see all

Anti-ME1 antibody 图像

  • Anti-ME1 antibody (ab87974) at 5 µg/ml + recombinant human ME1 protein with a proprietary tag (the immunogen) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 64 kDa
    Observed band size : 89 kDa (why is the actual band size different from the predicted?)
  • Anti-ME1 antibody (ab87974) at 5 µg/ml + HepG2 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 64 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ME1 antibody (ab87974) at 5 µg/ml

    Lane 1 : ME1 transfected lysate from 293T cell line
    Lane 2 : Non transfected lysate from 293T cell line

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
  • ab87974, at 0.5 µg/ml, staining ME1 in formalin fixed, paraffin embedded human tonsil tissue sections by Immunoperoxidase/Immunohistochemistry.
  • ab87974, detecting recombinant tagged ME1 by sandwich ELISA. The detection limit is approximately 1 ng/ml as a capture antibody.

Anti-ME1 antibody (ab87974)参考文献

ab87974 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab87974.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"