概述

  • 产品名称Anti-MDP1抗体
    参阅全部 MDP1 一抗
  • 描述
    兔多克隆抗体to MDP1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MARLPKLAVF DLDYTLWPFW VDTHVDPPFH KSSDGTVRDR RGQDVRLYPE) of Human MDP1 (NP_612485)

  • 阳性对照
    • HT1080 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab99033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IP Use at an assay dependent concentration.

靶标

Anti-MDP1 antibody 图像

  • Anti-MDP1 antibody (ab99033) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Predicted band size : 20 kDa

Anti-MDP1 antibody (ab99033)参考文献

ab99033 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HEK293 cells)
Specification HEK293 cells
Treatment Over expressed with MDP1 plasmid
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 37°C
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提交于 Apr 10 2015

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (HEK293 cells)
Specification HEK293 cells
Treatment over expressed with MDP1 plasmid
Total protein in input 100 µg
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提交于 Apr 10 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Staph. aureus
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Total protein in input 100 µg
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提交于 Sep 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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提交于 Sep 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (MCF7)
Total protein in input 100 µg
Immuno-precipitation step Other - sepharose beads
Specification MCF7
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提交于 May 16 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 May 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"