Anti-MDMX (phospho S403)抗体(ab70236)

概述

  • 产品名称Anti-MDMX (phospho S403)抗体
    参阅全部 MDMX 一抗
  • 描述
    兔多克隆抗体to MDMX (phospho S403)
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic phospho-peptide corresponding to the region around Serine 403 of Human MDMX (SwissProt O15151)

性能

应用

Our Abpromise guarantee covers the use of ab70236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/3000. Detects a band of approximately 80 kDa.

靶标

  • 功能Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
  • 组织特异性Expressed in all tissues tested with high levels in thymus.
  • 序列相似性Belongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.
  • 结构域Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
  • 翻译后修饰Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp781B1423 antibody
    • Double minute 4 antibody
    • Double minute 4 human homolog of p53 binding protein antibody
    • Double minute 4 protein antibody
    • HDMX antibody
    • MDM 4 antibody
    • Mdm2 like p53 binding protein antibody
    • Mdm2-like p53-binding protein antibody
    • MDM4 antibody
    • Mdm4 p53 binding protein homolog mouse antibody
    • Mdm4 protein antibody
    • MDM4 related protein 1 antibody
    • Mdm4 transformed 3T3 cell double minute 4 antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
    • MDM4_HUMAN antibody
    • Mdmx protein antibody
    • MGC132766 antibody
    • Mouse double minute 4 homolog antibody
    • Mouse double minute 4 human homolog of p53 binding protein antibody
    • MRP 1 antibody
    • MRP1 antibody
    • p53 binding protein antibody
    • p53 BINDING PROTEIN MDM4 antibody
    • p53-binding protein Mdm4 antibody
    • Protein Mdm4 antibody
    • Protein Mdmx antibody
    see all

Anti-MDMX (phospho S403) antibody 图像

  • All lanes : Anti-MDMX (phospho S403) antibody (ab70236) at 0.13 µg/ml

    Lane 1 : 293T whole cell lysate with HA tagged-MDMX S403A mutant and NCS (2hrs at 200 ng/ml)
    Lane 2 : 293T whole cell lysate with HA tagged-MDMX S403A mutant and NCS (0.5hrs at 200 ng/ml)
    Lane 3 : 293T whole cell lysate with HA tagged-MDMX S403A mutant
    Lane 4 : 293T whole cell lysate with HA tagged-MDMX (wild-type) and NCS (2hrs at 200 ng/ml)
    Lane 5 : 293T whole cell lysate with HA tagged-MDMX(wild-type) and NCS (0.5hrs at 200 ng/ml)
    Lane 6 : 293T whole cell lysate with HA tagged-MDMX (wild-type)
    Lane 7 : mock transfected 293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique

    Observed band size : 80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

Anti-MDMX (phospho S403) antibody (ab70236)参考文献

ab70236 has not yet been referenced specifically in any publications.

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