Anti-MDMX抗体[MDMX-82] (ab49993)


  • 产品名称
    参阅全部 MDMX 一抗
  • 描述
    小鼠单克隆抗体[MDMX-82] to MDMX
  • 经测试应用
    适用于: ELISA, IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Xenopus laevis, Chimpanzee, Opossum
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 93-106 of Human MDMX.



Our Abpromise guarantee covers the use of ab49993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.


  • 功能
    Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
  • 组织特异性
    Expressed in all tissues tested with high levels in thymus.
  • 序列相似性
    Belongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.
  • 结构域
    Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
  • 翻译后修饰
    Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp781B1423 antibody
    • Double minute 4 antibody
    • Double minute 4 human homolog of p53 binding protein antibody
    • Double minute 4 protein antibody
    • HDMX antibody
    • MDM 4 antibody
    • Mdm2 like p53 binding protein antibody
    • Mdm2-like p53-binding protein antibody
    • MDM4 antibody
    • Mdm4 p53 binding protein homolog mouse antibody
    • Mdm4 protein antibody
    • MDM4 related protein 1 antibody
    • Mdm4 transformed 3T3 cell double minute 4 antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
    • MDM4_HUMAN antibody
    • Mdmx protein antibody
    • MGC132766 antibody
    • Mouse double minute 4 homolog antibody
    • Mouse double minute 4 human homolog of p53 binding protein antibody
    • MRP 1 antibody
    • MRP1 antibody
    • p53 binding protein antibody
    • p53 BINDING PROTEIN MDM4 antibody
    • p53-binding protein Mdm4 antibody
    • Protein Mdm4 antibody
    • Protein Mdmx antibody
    see all

Anti-MDMX antibody [MDMX-82] 图像

  • Anti-MDMX antibody [MDMX-82] (ab49993) at 1/1000 dilution + Mouse myoblast cell line C2C12 whole cell lysate at 15 µg

    HRP-conjugated sheep anti-mouse IgG polyclonal at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 hour

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-MDMX antibody [MDMX-82] (ab49993)参考文献

This product has been referenced in:
  • Endo S  et al. Potent in vitro and in vivo antitumor effects of MDM2 inhibitor nutlin-3 in gastric cancer cells. Cancer Sci 102:605-13 (2011). WB ; Human . Read more (PubMed: 21205074) »
  • Marine JC & Jochemsen AG Mdmx as an essential regulator of p53 activity. Biochem Biophys Res Commun 331:750-60 (2005). Read more (PubMed: 15865931) »

See all 2 Publications for this product

Product Wall

Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Mouse Cell lysate - whole cell (mouse myoblast cell line C2C12)
mouse myoblast cell line C2C12
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Jul 25 2014

Thank you for your inquiry. The actual size was smaller than the predicted one, the reason can be thought that the post translational modification, e.g. phosphorylation, glycosylation etc. They increase the size of the protein. The 80 kDa band of ab1...

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