Anti-MDM2 (phospho S185)抗体(ab1094)


  • 产品名称
    Anti-MDM2 (phospho S185)抗体
    参阅全部 MDM2 一抗
  • 描述
    兔多克隆抗体to MDM2 (phospho S185)
  • 特异性
    Specific for phosphorylated MDM 2, giving a band in western blotting at approx 102 kD.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 177-198 of Mouse MDM2



Our Abpromise guarantee covers the use of ab1094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明
    WB: 1/1000 - 1/5000. Predicted molecular weight: 55 kDa.
    This product has been assayed by ELISA against 0.1µg of the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways.
    • 组织特异性
      Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
    • 疾病相关
      Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.
    • 序列相似性
      Belongs to the MDM2/MDM4 family.
      Contains 1 RanBP2-type zinc finger.
      Contains 1 RING-type zinc finger.
      Contains 1 SWIB domain.
    • 结构域
      Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.
    • 翻译后修饰
      Phosphorylated in response to ionizing radiation in an ATM-dependent manner.
      Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiquitinated by USP7; leading to stabilize it.
    • 细胞定位
      Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ACTFS antibody
      • Double minute 2 protein antibody
      • E3 ubiquitin-protein ligase Mdm2 antibody
      • Hdm 2 antibody
      • Hdm2 antibody
      • HDMX antibody
      • MDM 2 antibody
      • MDM2 antibody
      • MDM2 oncogene E3 ubiquitin protein ligase antibody
      • Mdm2 p53 E3 ubiquitin protein ligase homolog antibody
      • Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody
      • MDM2_HUMAN antibody
      • MDM2BP antibody
      • Mouse Double Minute 2 antibody
      • MTBP antibody
      • Murine Double Minute Chromosome 2 antibody
      • Oncoprotein Mdm2 antibody
      • p53 Binding Protein Mdm2 antibody
      • p53-binding protein Mdm2 antibody
      • Ubiquitin protein ligase E3 Mdm2 antibody
      • Ubiquitin protein ligase E3 Mdm2 antibody
      see all

    Anti-MDM2 (phospho S185) antibody 图像

    • Predicted band size : 55 kDa

      This image is courtesy of Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada

      Rabbit polyclonal to phospho MDM 2 (Ser 185) at 1/1000 on 293T cell extract shows a band at 102 kD.
      Cells were serum starved for 24 hours:

      Lane 1 : control
      Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min

    Anti-MDM2 (phospho S185) antibody (ab1094)参考文献

    ab1094 has not yet been referenced specifically in any publications.

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