Anti-MDH2抗体[2F5AF8] (ab110317)

概述

  • 产品名称Anti-MDH2抗体[2F5AF8]
    参阅全部 MDH2 一抗
  • 描述
    小鼠单克隆抗体[2F5AF8] to MDH2
  • 经测试应用适用于: ICC/IF, In-Cell ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
  • 免疫原

    Tissue, cells or virus corresponding to Pig MDH2. Purified protein from porcine heart.

  • 阳性对照
    • Fibroblast cells Human heart homogenate Rat liver homogenate Mouse liver homogenate HepG2 whole cell lysate
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml.
IP Use at an assay dependent dilution.

靶标

Anti-MDH2 antibody [2F5AF8] 图像

  • Immunocytochemistry image of stained fibroblast cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the ab110317 (1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix
  • ab110317 pulls down the 33kDa MDH2 protein from Human heart homogenate (1), Rat liver homogenate (2), Mouse liver homogenate (3) and HepG2 whole cell lysate (4). The identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

Anti-MDH2 antibody [2F5AF8] (ab110317)参考文献

ab110317 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"