重组Anti-MDH1抗体[EPR13597(B)] - C-terminal (ab180152)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13597(B)] to MDH1 - C-terminal
- Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MDH1抗体[EPR13597(B)] - C-terminal
参阅全部 MDH1 一抗 -
描述
兔单克隆抗体[EPR13597(B)] to MDH1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat, HeLa and HepG2 whole cell lysate (ab7900); HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13597(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180152于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
1/250.
|
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IP |
1/50.
|
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WB |
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
|
说明 |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/250. |
IP
1/50. |
WB
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
靶标
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序列相似性
Belongs to the LDH/MDH superfamily. MDH type 2 family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4190 Human
- Entrez Gene: 17449 Mouse
- Entrez Gene: 24551 Rat
- Omim: 154200 Human
- SwissProt: P40925 Human
- SwissProt: P14152 Mouse
- SwissProt: O88989 Rat
- Unigene: 526521 Human
see all -
别名
- cytoplasmic antibody
- Cytosolic malate dehydrogenase antibody
- Diiodophenylpyruvate reductase antibody
see all
图片
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All lanes : Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa -
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI.
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Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180152 at 1/50 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/20 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (10)
ab180152 被引用在 10 文献中.
- Marsilio S et al. Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma. J Vet Intern Med 35:179-189 (2021). PubMed: 33471936
- Ying M et al. Lactate and glutamine support NADPH generation in cancer cells under glucose deprived conditions. Redox Biol 46:102065 (2021). PubMed: 34293554
- Nelson BS et al. Tissue of origin dictates GOT1 dependence and confers synthetic lethality to radiotherapy. Cancer Metab 8:1 (2020). PubMed: 31908776
- Labuschagne CF et al. Cell Clustering Promotes a Metabolic Switch that Supports Metastatic Colonization. Cell Metab 30:720-734.e5 (2019). PubMed: 31447323
- Zhang W et al. Ketamine exerts a protective role in a cell-based model of major depressive disorder via the inhibition of apoptosis and inflammation and activation of the Krebs cycle. Bosn J Basic Med Sci N/A:N/A (2019). PubMed: 31215856
- Tajan M et al. A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3. Cell Metab 28:721-736.e6 (2018). PubMed: 30122553
- Gaude E et al. NADH Shuttling Couples Cytosolic Reductive Carboxylation of Glutamine with Glycolysis in Cells with Mitochondrial Dysfunction. Mol Cell 69:581-593.e7 (2018). PubMed: 29452638
- Bao Y et al. Carnosine Inhibits the Proliferation of Human Cervical Gland Carcinoma Cells Through Inhibiting Both Mitochondrial Bioenergetics and Glycolysis Pathways and Retarding Cell Cycle Progression. Integr Cancer Ther 17:80-91 (2018). PubMed: 28008780
- Pace MC et al. Changes in proteome solubility indicate widespread proteostatic disruption in mouse models of neurodegenerative disease. Acta Neuropathol 136:919-938 (2018). PubMed: 30140941
- Hanse EA et al. Cytosolic malate dehydrogenase activity helps support glycolysis in actively proliferating cells and cancer. Oncogene 36:3915-3924 (2017). PubMed: 28263970