Anti-MDH1抗体[2B11-B7] (ab55528)

概述

  • 产品名称Anti-MDH1抗体[2B11-B7]
    参阅全部 MDH1 一抗
  • 描述
    小鼠单克隆抗体[2B11-B7] to MDH1
  • 经测试应用适用于: WB, IP, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-335 of Human Malate Dehydrogenase 1 (MDH1).

性能

应用

Our Abpromise guarantee covers the use of ab55528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 序列相似性Belongs to the LDH/MDH superfamily. MDH type 2 family.
  • 翻译后修饰ISGylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cytoplasmic antibody
    • Cytosolic malate dehydrogenase antibody
    • Diiodophenylpyruvate reductase antibody
    • Malate dehydrogenase 1, NAD (soluble) antibody
    • Malate dehydrogenase antibody
    • Malate dehydrogenase cytoplasmic antibody
    • MDH s antibody
    • mdh1 antibody
    • MDHA antibody
    • MDHC_HUMAN antibody
    • MDHs antibody
    • MGC:1375 antibody
    • MOR2 antibody
    • Soluble malate dehydrogenase antibody
    see all

Anti-MDH1 antibody [2B11-B7] 图像



  • Predicted band size : 36 kDa
  • Overlay histogram showing HL60 cells stained with ab55528 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55528, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Detection of MDH1 by Immunoprecipitation. ab55528 used at 5µl/mg for 500µg of Mouse whole cell lysate from Hippocampal neurol cells for IP using Dynabeads for 16 hours at 4 degrees (in PBST). Immunoprecipitated MAD2 was blotted with another antibody HRP-congugated Goat anti-mouse Polyclonal at 1/5000. Detection: Chemiluminescence with an exposure time of 1 minute. Band size 36kDa.

    See Abreview

Anti-MDH1 antibody [2B11-B7] (ab55528)参考文献

ab55528 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (neuronal primary cultures)
Specification neuronal primary cultures
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton x-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
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提交于 Sep 11 2012

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (hippocampal neuronal cell)
Total protein in input 500 µg
Specification hippocampal neuronal cell
Immuno-precipitation step Other - dynabead
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提交于 Aug 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (neuronal)
Loading amount 50 µg
Specification neuronal
Gel Running Conditions Non-reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
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提交于 Aug 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"