概述

  • 产品名称
  • 描述
    兔多克隆抗体to MDFIC
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the N terminal region of Human MDFIC (NP_951038.1).

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab93224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 37 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      MDFIC is a cellular factor capable of modulating the expression of cellular and viral promoters. Its synthesis is controlled at the translational level by two different codons, an ATG and an upstream non-ATG translational initiator, allowing the production of two protein isoforms that present different subcellular localizations, p32 (isoform 2) being mainly distributed throughout the cytoplasm, whereas p40 (isoform 1) is targeted to the nucleolus. MDFIC is known to down-regulate Tat-dependent transcription of HIV-1 LTR by interacting with HIV-1 Tat and Rev proteins and impairing their nuclear import (possibly by rendering the NLS domains inaccessible to importin-beta) and stimulate activation of human T-cell leukaemia virus type I (HTLV-I) LTR. In human cells, MDFIC binds to the axin complex, resulting in an increase in the level of free beta-catenin; it affects axin regulation of the WNT and JNK signalling pathways.
    • 细胞定位
      Cytoplasmic and Nuclear
    • 数据库链接
    • 别名
      • HIC antibody
      • Human I mfa domain containing protein antibody
      • Human I-mfa domain-Containing protein antibody
      • I mfa domain containing protein antibody
      • MyoD family inhibitor domain containing protein antibody
      • MyoD family inhibitor domain-containing protein antibody
      • p32 antibody
      • p40 antibody
      see all

    图片

    • Anti-MDFIC antibody (ab93224) at 1/100 dilution + HeLa cell lysate at 35 µg

      Predicted band size : 37 kDa

    实验方案

    文献

    ab93224 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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