Anti-MDC1 (phospho T4)抗体(ab35967)

概述

  • 产品名称Anti-MDC1 (phospho T4)抗体
    参阅全部 MDC1 一抗
  • 描述
    兔多克隆抗体to MDC1 (phospho T4)
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MDC1, phosphorylated at T4.

    (Peptide available as ab36513.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Cell Lysates: HepG2 Nuclear - IFN gamma treated HeLa Whole Cell - UV Irradiated (data not shown)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab35967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 287 kDa (predicted molecular weight: 227 kDa).

靶标

  • 功能Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • 组织特异性Highly expressed in testis.
  • 序列相似性Contains 2 BRCT domains.
    Contains 1 FHA domain.
  • 结构域Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • 翻译后修饰Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • 细胞定位Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homologue to Drosophila photoreceptor protein calphotin antibody
    • MDC 1 antibody
    • Mdc1 antibody
    • MDC1_HUMAN antibody
    • Mediation of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint protein 1 antibody
    • NFBD 1 antibody
    • NFBD1 antibody
    • Nuclear factor with BRCT domains 1 antibody
    • Nuclear Factor with BRCT Domains Protein 1 antibody
    see all

Anti-MDC1 (phospho T4) antibody 图像

  • ab35967 staining MDC1 (phospho T4) in HeLa cells (untreated or treated with 10 Gy IR) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour. Samples were incubated with primary antibody (1/200 in 5% BSA) for 1 hour. A FITC-conjugated donkey anti-rabbit IgG polyclonal (1/150) was used as the secondary antibody.

    See Abreview

  • This antibody gave a positive result in ELISA against the immunizing peptide (blue line, ab36513). It gave a negative result in ELISA against the non-modified equivalent peptide (yellow line, ab36514). This indicates that it is specific for the modified peptide.
        

Anti-MDC1 (phospho T4) antibody (ab35967)参考文献

ab35967 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Treatment + 5 Gy IR
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Jul 08 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - Triton X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 08 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HCT116 (colorectal))
Specification HCT116 (colorectal)
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Jan 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"