概述

  • 产品名称Anti-MDC1抗体
    参阅全部 MDC1 一抗
  • 描述
    兔多克隆抗体to MDC1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    RQESDKNGESASPERDRE

    , corresponding to amino acids 871-888 of Human MDC1.

  • 阳性对照
    • Jurkat whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab13858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 250 kDa).

靶标

  • 功能Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • 组织特异性Highly expressed in testis.
  • 序列相似性Contains 2 BRCT domains.
    Contains 1 FHA domain.
  • 结构域Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • 翻译后修饰Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • 细胞定位Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homologue to Drosophila photoreceptor protein calphotin antibody
    • MDC 1 antibody
    • Mdc1 antibody
    • MDC1_HUMAN antibody
    • Mediation of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint protein 1 antibody
    • NFBD 1 antibody
    • NFBD1 antibody
    • Nuclear factor with BRCT domains 1 antibody
    • Nuclear Factor with BRCT Domains Protein 1 antibody
    see all

Anti-MDC1 antibody 图像



  • Predicted band size : 250 kDa


    Western blot analysis of MDC1 in 30 ugs of Jurkat cell lysate using ab 13858 at 1/1000 dilution.

Anti-MDC1 antibody (ab13858)参考文献

This product has been referenced in:
  • Ismail IA  et al. Genistein-induced neuronal apoptosis and G2/M cell cycle arrest is associated with MDC1 up-regulation and PLK1 down-regulation. Eur J Pharmacol 575:12-20 (2007). WB ; Human . Read more (PubMed: 17706963) »

See 1 Publication for this product

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